TY - JOUR
T1 - Uncharacterized Escherichia coli proteins YdjA and YhjY are related to biohydrogen production
AU - Mohd Yusoff, Mohd Zulkhairi
AU - Maeda, Toshinari
AU - Sanchez-Torres, Viviana
AU - Ogawa, Hiroaki I.
AU - Shirai, Yoshihito
AU - Hassan, Mohd Ali
AU - Wood, Thomas K.
N1 - Funding Information:
The authors would like to acknowledge the National of Institute of Genetics (Japan) for providing several Keio mutants and ASKA strains, the National Science Foundation for supporting this research (CBET-0753702), and the Japanese Science Society and the Japan Society for the Promotion of Science for supporting this research through the Sasakawa Scientific Research Grant ( 24-521 ) and Grant-In-Aid for Young Scientists (B) ( 22780070 ), respectively.
PY - 2012/12
Y1 - 2012/12
N2 - Biohydrogen has gained importance as an alternative energy source, and advances in molecular biology and biotechnology have raised the quality and efficiency of biohydrogen production from various microorganisms and substrates. Here, Escherichia coli proteins YdjA and YhjY have been identified as essential in biohydrogen production from glucose. The mutations ydjA and yhjY reduced biohydrogen productivity compared to the parent strain from 40 to 4 and 29 μmol/mg protein, respectively. Through transcription analysis, it was determined that YdjA and YhjY are positive effectors of the FHL complex since their inactivation repressed fhlA. In addition, the FHL expression of the repressor gene, hycA, increased for the ydjA mutant, so YdjA reduces transcription of the HycA repressor. Hence, two new proteins have been identified that are important for biohydrogen production.
AB - Biohydrogen has gained importance as an alternative energy source, and advances in molecular biology and biotechnology have raised the quality and efficiency of biohydrogen production from various microorganisms and substrates. Here, Escherichia coli proteins YdjA and YhjY have been identified as essential in biohydrogen production from glucose. The mutations ydjA and yhjY reduced biohydrogen productivity compared to the parent strain from 40 to 4 and 29 μmol/mg protein, respectively. Through transcription analysis, it was determined that YdjA and YhjY are positive effectors of the FHL complex since their inactivation repressed fhlA. In addition, the FHL expression of the repressor gene, hycA, increased for the ydjA mutant, so YdjA reduces transcription of the HycA repressor. Hence, two new proteins have been identified that are important for biohydrogen production.
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U2 - 10.1016/j.ijhydene.2012.08.115
DO - 10.1016/j.ijhydene.2012.08.115
M3 - Article
AN - SCOPUS:84868208556
SN - 0360-3199
VL - 37
SP - 17778
EP - 17787
JO - International Journal of Hydrogen Energy
JF - International Journal of Hydrogen Energy
IS - 23
ER -