TY - JOUR
T1 - Unlinked genetic loci control the reduced transcription of aminopeptidase N 1 and 3 in the European corn borer and determine tolerance to Bacillus thuringiensis Cry1Ab toxin
AU - Coates, Brad S.
AU - Sumerford, Douglas V.
AU - Siegfried, Blair D.
AU - Hellmich, Richard L.
AU - Abel, Craig A.
N1 - Funding Information:
This research was a joint contribution from the United States Department of Agriculture (USDA), Agricultural Research Service (ARS) (CRIS Project 3625-22000-017-00), the Iowa Agriculture and Home Economics Experiment Station, Ames, IA (Project 3543), and the University of Nebraska. Funding also was provided by a USDA , National Institute of Food and Agriculture (NIFA) (Award Number 2009-05246). The authors wish to thank USDA Technicians Bob Gunnarson, Cindy Backus and Jean Dyer for contributions to genotyping and insect rearing procedures. This article reports the results of research only. Mention of a proprietary product does not constitute an endorsement or a recommendation by USDA, Iowa State University or the University of Nebraska for its use. Appendix A
PY - 2013/12
Y1 - 2013/12
N2 - Transgenic expression of Bacillus thuringiensis (Bt) crystalline (Cry) toxins by crop plants result in reduced insect feeding damage, but sustainability is threatened by the development of resistance traits in target insect populations. We investigated Bt toxin resistance trait in a laboratory colony of the European corn borer, Ostrinia nubilalis, selected for increased survival when exposed to Cry1Ab and correlated survival on Cry1Ab toxin with a constitutive ~146.2±17.3-fold reduction in midgut aminopeptidase N1 (apn1) transcript levels. A 7.1±1.9-fold reduction apn3 transcript level was also correlated with Cry1Ab resistance. Quantitative trait locus (QTL) mapping identified a single major genome region controlling Cry1Ab resistance on linkage group 24 (LG24), and a minor QTL on LG27. Both QTL were independent of apn1 and apn3 loci on LG02. Positional mapping identified genetic markers that may assist in the identification of causal gene(s) within QTL intervals. This study indicates that genetic factor(s) may act in trans to reduce both apn1 and apn3 expression in Cry1Ab resistant O.nubilalis larvae, and suggest that gene regulatory pathways can influence Bt resistance traits. These findings show that gene interactions (epistasis) may influence Bt resistance in target insect populations.
AB - Transgenic expression of Bacillus thuringiensis (Bt) crystalline (Cry) toxins by crop plants result in reduced insect feeding damage, but sustainability is threatened by the development of resistance traits in target insect populations. We investigated Bt toxin resistance trait in a laboratory colony of the European corn borer, Ostrinia nubilalis, selected for increased survival when exposed to Cry1Ab and correlated survival on Cry1Ab toxin with a constitutive ~146.2±17.3-fold reduction in midgut aminopeptidase N1 (apn1) transcript levels. A 7.1±1.9-fold reduction apn3 transcript level was also correlated with Cry1Ab resistance. Quantitative trait locus (QTL) mapping identified a single major genome region controlling Cry1Ab resistance on linkage group 24 (LG24), and a minor QTL on LG27. Both QTL were independent of apn1 and apn3 loci on LG02. Positional mapping identified genetic markers that may assist in the identification of causal gene(s) within QTL intervals. This study indicates that genetic factor(s) may act in trans to reduce both apn1 and apn3 expression in Cry1Ab resistant O.nubilalis larvae, and suggest that gene regulatory pathways can influence Bt resistance traits. These findings show that gene interactions (epistasis) may influence Bt resistance in target insect populations.
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U2 - 10.1016/j.ibmb.2013.09.003
DO - 10.1016/j.ibmb.2013.09.003
M3 - Article
C2 - 24121099
AN - SCOPUS:84886405954
SN - 0965-1748
VL - 43
SP - 1152
EP - 1160
JO - Insect Biochemistry and Molecular Biology
JF - Insect Biochemistry and Molecular Biology
IS - 12
ER -