Unnatural translation initiation

John W. Tomsho; Stephen J. Benkovic

doi:10.1021/cb8000233

Unnatural translation initiation

John W. Tomsho, Stephen J. Benkovic

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Protein translation in nature always begins with an initiator transfer RNA (tRNA) carrying the amino acid methionine. This was circumvented in vitro with a reconstituted translation system utilizing initiator tRNA synthetically mischarged with the other natural amino acids. In addition, it was determined that this system could accommodate these non-methionine amino acids containing various N-α-acyl groups, many of which are useful for post-translational modification such as peptide cyclization.

Original language	English (US)
Pages (from-to)	87-88
Number of pages	2
Journal	ACS chemical biology
Volume	3
Issue number	2
DOIs	https://doi.org/10.1021/cb8000233
State	Published - Feb 2008

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine

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10.1021/cb8000233

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title = "Unnatural translation initiation",

abstract = "Protein translation in nature always begins with an initiator transfer RNA (tRNA) carrying the amino acid methionine. This was circumvented in vitro with a reconstituted translation system utilizing initiator tRNA synthetically mischarged with the other natural amino acids. In addition, it was determined that this system could accommodate these non-methionine amino acids containing various N-α-acyl groups, many of which are useful for post-translational modification such as peptide cyclization.",

author = "Tomsho, {John W.} and Benkovic, {Stephen J.}",

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AB - Protein translation in nature always begins with an initiator transfer RNA (tRNA) carrying the amino acid methionine. This was circumvented in vitro with a reconstituted translation system utilizing initiator tRNA synthetically mischarged with the other natural amino acids. In addition, it was determined that this system could accommodate these non-methionine amino acids containing various N-α-acyl groups, many of which are useful for post-translational modification such as peptide cyclization.

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Unnatural translation initiation

Abstract

All Science Journal Classification (ASJC) codes

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