Unnatural translation initiation

John W. Tomsho; Stephen J. Benkovic

doi:10.1021/cb8000233

Unnatural translation initiation

John W. Tomsho
, Stephen J. Benkovic

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Protein translation in nature always begins with an initiator transfer RNA (tRNA) carrying the amino acid methionine. This was circumvented in vitro with a reconstituted translation system utilizing initiator tRNA synthetically mischarged with the other natural amino acids. In addition, it was determined that this system could accommodate these non-methionine amino acids containing various N-α-acyl groups, many of which are useful for post-translational modification such as peptide cyclization.

Original language	English (US)
Pages (from-to)	87-88
Number of pages	2
Journal	ACS chemical biology
Volume	3
Issue number	2
DOIs	https://doi.org/10.1021/cb8000233
State	Published - Feb 2008

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine

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10.1021/cb8000233

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title = "Unnatural translation initiation",

abstract = "Protein translation in nature always begins with an initiator transfer RNA (tRNA) carrying the amino acid methionine. This was circumvented in vitro with a reconstituted translation system utilizing initiator tRNA synthetically mischarged with the other natural amino acids. In addition, it was determined that this system could accommodate these non-methionine amino acids containing various N-α-acyl groups, many of which are useful for post-translational modification such as peptide cyclization.",

author = "Tomsho, \{John W.\} and Benkovic, \{Stephen J.\}",

year = "2008",

month = feb,

doi = "10.1021/cb8000233",

language = "English (US)",

volume = "3",

pages = "87--88",

journal = "ACS chemical biology",

issn = "1554-8929",

publisher = "American Chemical Society",

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AU - Benkovic, Stephen J.

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N2 - Protein translation in nature always begins with an initiator transfer RNA (tRNA) carrying the amino acid methionine. This was circumvented in vitro with a reconstituted translation system utilizing initiator tRNA synthetically mischarged with the other natural amino acids. In addition, it was determined that this system could accommodate these non-methionine amino acids containing various N-α-acyl groups, many of which are useful for post-translational modification such as peptide cyclization.

AB - Protein translation in nature always begins with an initiator transfer RNA (tRNA) carrying the amino acid methionine. This was circumvented in vitro with a reconstituted translation system utilizing initiator tRNA synthetically mischarged with the other natural amino acids. In addition, it was determined that this system could accommodate these non-methionine amino acids containing various N-α-acyl groups, many of which are useful for post-translational modification such as peptide cyclization.

UR - https://www.scopus.com/pages/publications/41049096234

UR - https://www.scopus.com/pages/publications/41049096234#tab=citedBy

U2 - 10.1021/cb8000233

DO - 10.1021/cb8000233

M3 - Article

C2 - 18278847

AN - SCOPUS:41049096234

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VL - 3

SP - 87

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JO - ACS chemical biology

JF - ACS chemical biology

IS - 2

ER -

Unnatural translation initiation

Abstract

All Science Journal Classification (ASJC) codes

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