TY - JOUR
T1 - Use of a conserved consensus oligomer in the identification of cytochrome P-450 mRNAs
AU - Hassett, Christopher
AU - Omiecinski, Curtis J.
PY - 1987/12/16
Y1 - 1987/12/16
N2 - A region of high nucleic acid conservation present in many members of the phenobarbital (PB) family of cytochromes P-450 (P-450s) was targeted for the construction of an 18 base, eight-fold degenerate, consensus oligomer (the PB-mer). When utilized to screen a rabbit liver cDNA library, the PB-mer enabled the isolation of P-450 cDNAs. One isolate, designated pP-450 1a, was characterized by nucleotide sequence, as well as Northern and Southern blot analyses and was demonstrated to be a novel form. Primary nucleotide sequence indicates that this cDNA is highly homologous to several P-450 cDNAs, but only in the protein coding region. The 3′-noncoding segment of P-450 1a is longer and diverges markedly from known P-450 cDNAs in the rabbit PB family.
AB - A region of high nucleic acid conservation present in many members of the phenobarbital (PB) family of cytochromes P-450 (P-450s) was targeted for the construction of an 18 base, eight-fold degenerate, consensus oligomer (the PB-mer). When utilized to screen a rabbit liver cDNA library, the PB-mer enabled the isolation of P-450 cDNAs. One isolate, designated pP-450 1a, was characterized by nucleotide sequence, as well as Northern and Southern blot analyses and was demonstrated to be a novel form. Primary nucleotide sequence indicates that this cDNA is highly homologous to several P-450 cDNAs, but only in the protein coding region. The 3′-noncoding segment of P-450 1a is longer and diverges markedly from known P-450 cDNAs in the rabbit PB family.
UR - http://www.scopus.com/inward/record.url?scp=0023604757&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023604757&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(87)90370-6
DO - 10.1016/0006-291X(87)90370-6
M3 - Article
C2 - 3426576
AN - SCOPUS:0023604757
SN - 0006-291X
VL - 149
SP - 326
EP - 333
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -