Use of a conserved consensus oligomer in the identification of cytochrome P-450 mRNAs

Christopher Hassett; Curtis J. Omiecinski

doi:10.1016/0006-291X(87)90370-6

Use of a conserved consensus oligomer in the identification of cytochrome P-450 mRNAs

Christopher Hassett
, Curtis J. Omiecinski

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A region of high nucleic acid conservation present in many members of the phenobarbital (PB) family of cytochromes P-450 (P-450s) was targeted for the construction of an 18 base, eight-fold degenerate, consensus oligomer (the PB-mer). When utilized to screen a rabbit liver cDNA library, the PB-mer enabled the isolation of P-450 cDNAs. One isolate, designated pP-450 1a, was characterized by nucleotide sequence, as well as Northern and Southern blot analyses and was demonstrated to be a novel form. Primary nucleotide sequence indicates that this cDNA is highly homologous to several P-450 cDNAs, but only in the protein coding region. The 3′-noncoding segment of P-450 1a is longer and diverges markedly from known P-450 cDNAs in the rabbit PB family.

Original language	English (US)
Pages (from-to)	326-333
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	149
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(87)90370-6
State	Published - Dec 16 1987

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(87)90370-6

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title = "Use of a conserved consensus oligomer in the identification of cytochrome P-450 mRNAs",

abstract = "A region of high nucleic acid conservation present in many members of the phenobarbital (PB) family of cytochromes P-450 (P-450s) was targeted for the construction of an 18 base, eight-fold degenerate, consensus oligomer (the PB-mer). When utilized to screen a rabbit liver cDNA library, the PB-mer enabled the isolation of P-450 cDNAs. One isolate, designated pP-450 1a, was characterized by nucleotide sequence, as well as Northern and Southern blot analyses and was demonstrated to be a novel form. Primary nucleotide sequence indicates that this cDNA is highly homologous to several P-450 cDNAs, but only in the protein coding region. The 3′-noncoding segment of P-450 1a is longer and diverges markedly from known P-450 cDNAs in the rabbit PB family.",

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AU - Omiecinski, Curtis J.

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Use of a conserved consensus oligomer in the identification of cytochrome P-450 mRNAs

Abstract

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