Use of a microarray to assess the distribution of plasmid and chromosomal virulence genes in strains of enteroaggregative Escherichia coli

Claire Jenkins; Carola Van Ijperen; Edward G. Dudley; Henrik Chart; Geraldine A. Willshaw; Tom Cheasty; Henry R. Smith; James P. Nataro

doi:10.1016/j.femsle.2005.09.040

Use of a microarray to assess the distribution of plasmid and chromosomal virulence genes in strains of enteroaggregative Escherichia coli

Claire Jenkins, Carola Van Ijperen, Edward G. Dudley, Henrik Chart, Geraldine A. Willshaw, Tom Cheasty, Henry R. Smith, James P. Nataro

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Abstract

A DNA microarray was used to analyze the distribution of plasmid and chromosomal genes among strains of enteroaggregative Escherichia coli (EAEC) isolated from a prospective diarrhoea surveillance study in the United Kingdom. Target genes were extracted from existing databases and from the genome sequence of prototype EAEC strain 042. We found that strains exhibiting the aggregative adherence (AA) phenotype could be broadly divided into two groups depending upon whether they harboured genes from the EAEC virulence plasmid (pAA) and a set of chromosomal genes found in EAEC strain 042. Several chromosomal loci were inherited en bloc, and were more common in strains which we designated Group 1; genes at the pheU locus were particularly conserved. Genes encoded on the pAA plasmid and those under control of the master regulator AggR were also concentrated in the Group 1 EAEC. A gene encoding a type 1 pilin allele was detected more frequently in Group 2 EAEC. Our data suggest that strains previously designated as typical EAEC harbour a large number of conserved plasmid and chromosomal loci, further illuminating a package of virulence genes common to the most important EAEC.

Original language	English (US)
Pages (from-to)	119-124
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	253
Issue number	1
DOIs	https://doi.org/10.1016/j.femsle.2005.09.040
State	Published - Dec 1 2005

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Microbiology
Molecular Biology
Genetics

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10.1016/j.femsle.2005.09.040

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title = "Use of a microarray to assess the distribution of plasmid and chromosomal virulence genes in strains of enteroaggregative Escherichia coli",

abstract = "A DNA microarray was used to analyze the distribution of plasmid and chromosomal genes among strains of enteroaggregative Escherichia coli (EAEC) isolated from a prospective diarrhoea surveillance study in the United Kingdom. Target genes were extracted from existing databases and from the genome sequence of prototype EAEC strain 042. We found that strains exhibiting the aggregative adherence (AA) phenotype could be broadly divided into two groups depending upon whether they harboured genes from the EAEC virulence plasmid (pAA) and a set of chromosomal genes found in EAEC strain 042. Several chromosomal loci were inherited en bloc, and were more common in strains which we designated Group 1; genes at the pheU locus were particularly conserved. Genes encoded on the pAA plasmid and those under control of the master regulator AggR were also concentrated in the Group 1 EAEC. A gene encoding a type 1 pilin allele was detected more frequently in Group 2 EAEC. Our data suggest that strains previously designated as typical EAEC harbour a large number of conserved plasmid and chromosomal loci, further illuminating a package of virulence genes common to the most important EAEC.",

author = "Claire Jenkins and {Van Ijperen}, Carola and Dudley, {Edward G.} and Henrik Chart and Willshaw, {Geraldine A.} and Tom Cheasty and Smith, {Henry R.} and Nataro, {James P.}",

note = "Funding Information: This work was funded by the Food Standards Agency under a grant awarded to Dr. Henrik Chart, and by US PHS award AI 33096 to JPN. This paper is dedicated to the memory of Henry Smith, Ph.D. ",

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AU - Willshaw, Geraldine A.

AU - Cheasty, Tom

AU - Smith, Henry R.

AU - Nataro, James P.

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Use of a microarray to assess the distribution of plasmid and chromosomal virulence genes in strains of enteroaggregative Escherichia coli

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