This overview unit discusses fluorescence spectroscopy as a tool for studying RNA folding. Ribozymes and oligonucleotides can be labeled with a fluorescent probe and analyzed to give information about both slow and fast kinetic processes with real-time data acquisition. The unit discusses the advantages and disadvantages of various pendant probes and nucleotide analogs, the analytical methods that can be used, instrument setup, control experiments, and a variety of kinetic experiments that can be performed, such as determination of rate constants.
|Original language||English (US)|
|Journal||Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]|
|State||Published - Jan 1 2002|
All Science Journal Classification (ASJC) codes
- Organic Chemistry