TY - JOUR
T1 - Using time-lapse fluorescence microscopy to study gene regulation
AU - Zou, Fan
AU - Bai, Lu
N1 - Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/4/15
Y1 - 2019/4/15
N2 - Time-lapse fluorescence microscopy is a powerful tool to study gene regulation. By probing fluorescent signals in single cells over extended period of time, this method can be used to study the dynamics, noise, movement, memory, inheritance, and coordination, of gene expression during cell growth, development, and differentiation. In combination with a flow-cell device, it can also measure gene regulation by external stimuli. Due to the single cell nature and the spatial/temporal capacity, this method can often provide information that is hard to get using other methods. Here, we review the standard experimental procedures and new technical developments in this field.
AB - Time-lapse fluorescence microscopy is a powerful tool to study gene regulation. By probing fluorescent signals in single cells over extended period of time, this method can be used to study the dynamics, noise, movement, memory, inheritance, and coordination, of gene expression during cell growth, development, and differentiation. In combination with a flow-cell device, it can also measure gene regulation by external stimuli. Due to the single cell nature and the spatial/temporal capacity, this method can often provide information that is hard to get using other methods. Here, we review the standard experimental procedures and new technical developments in this field.
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U2 - 10.1016/j.ymeth.2018.12.010
DO - 10.1016/j.ymeth.2018.12.010
M3 - Review article
C2 - 30599195
AN - SCOPUS:85059436993
SN - 1046-2023
VL - 159-160
SP - 138
EP - 145
JO - Methods
JF - Methods
ER -