Utility of multiplex reverse transcriptase-polymerase chain reaction for diagnosis and serotypic characterization of dengue and chikungunya viruses in clinical samples

Baijayantimala Mishra; Mirnalini Sharma; Sujit Kumar Pujhari; Radha Kanta Ratho; DVR V.R.S. Gopal; CVM Naresh Kumar; Gita Sarangi; Nirupama Chayani; Subhash C. Varma

doi:10.1016/j.diagmicrobio.2011.06.020

Utility of multiplex reverse transcriptase-polymerase chain reaction for diagnosis and serotypic characterization of dengue and chikungunya viruses in clinical samples

Baijayantimala Mishra, Mirnalini Sharma, Sujit Kumar Pujhari, Radha Kanta Ratho, DVR V.R.S. Gopal, CVM Naresh Kumar, Gita Sarangi, Nirupama Chayani, Subhash C. Varma

Entomology

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22 Scopus citations

Abstract

The reemergence of chikungunya virus (CHIKV) has compounded the already existing dengue problem because of clinical similarities and common vector, demanding the need for a rapid and specific diagnosis. Thus, dengue chikungunya multiplex reverse transcriptase-polymerase chain reaction (DCmRT-PCR) was developed and validated for simultaneous detection of dengue and chikungunya viral infections and its utility in virus serotyping. Blood samples from 97 suspected dengue and chikungunya cases and 10 healthy controls were subjected to dengue and chikungunya conventional RT-PCR and DCmRT-PCR. Thirty-one of 97 samples were positive for dengue or chikungunya viral RNA by RT-PCR and DCmRT-PCR with 100% concordance. DCmRT-PCR products were cycle sequenced. Seven dengue virus strains were clustered within genotype III of DENV-3 and 4 within genotype III of DENV-1, whereas chikungunya sequences were clustered within the Central/East African genotype. DCmRT-PCR was found to be a potential rapid test for simultaneous detection of dengue and CHIKV in clinical samples along with dengue serotyping.

Original language	English (US)
Pages (from-to)	118-125
Number of pages	8
Journal	Diagnostic Microbiology and Infectious Disease
Volume	71
Issue number	2
DOIs	https://doi.org/10.1016/j.diagmicrobio.2011.06.020
State	Published - Oct 2011

All Science Journal Classification (ASJC) codes

Microbiology (medical)
Infectious Diseases

UN SDGs

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10.1016/j.diagmicrobio.2011.06.020

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Mishra, B., Sharma, M., Pujhari, S. K., Ratho, R. K., Gopal, DVR. V. R. S., Kumar, CVM. N., Sarangi, G., Chayani, N., & Varma, S. C. (2011). Utility of multiplex reverse transcriptase-polymerase chain reaction for diagnosis and serotypic characterization of dengue and chikungunya viruses in clinical samples. Diagnostic Microbiology and Infectious Disease, 71(2), 118-125. https://doi.org/10.1016/j.diagmicrobio.2011.06.020

@article{c1cd8633bf7549388e5312119f48683f,

title = "Utility of multiplex reverse transcriptase-polymerase chain reaction for diagnosis and serotypic characterization of dengue and chikungunya viruses in clinical samples",

abstract = "The reemergence of chikungunya virus (CHIKV) has compounded the already existing dengue problem because of clinical similarities and common vector, demanding the need for a rapid and specific diagnosis. Thus, dengue chikungunya multiplex reverse transcriptase-polymerase chain reaction (DCmRT-PCR) was developed and validated for simultaneous detection of dengue and chikungunya viral infections and its utility in virus serotyping. Blood samples from 97 suspected dengue and chikungunya cases and 10 healthy controls were subjected to dengue and chikungunya conventional RT-PCR and DCmRT-PCR. Thirty-one of 97 samples were positive for dengue or chikungunya viral RNA by RT-PCR and DCmRT-PCR with 100% concordance. DCmRT-PCR products were cycle sequenced. Seven dengue virus strains were clustered within genotype III of DENV-3 and 4 within genotype III of DENV-1, whereas chikungunya sequences were clustered within the Central/East African genotype. DCmRT-PCR was found to be a potential rapid test for simultaneous detection of dengue and CHIKV in clinical samples along with dengue serotyping.",

author = "Baijayantimala Mishra and Mirnalini Sharma and Pujhari, {Sujit Kumar} and Ratho, {Radha Kanta} and Gopal, {DVR V.R.S.} and Kumar, {CVM Naresh} and Gita Sarangi and Nirupama Chayani and Varma, {Subhash C.}",

note = "Funding Information: The authors acknowledge the financial grant received from the Department of Science and Technology (DST), New Delhi, India, for this work.",

year = "2011",

month = oct,

doi = "10.1016/j.diagmicrobio.2011.06.020",

language = "English (US)",

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Mishra, B, Sharma, M, Pujhari, SK, Ratho, RK, Gopal, DVRVRS, Kumar, CVMN, Sarangi, G, Chayani, N & Varma, SC 2011, 'Utility of multiplex reverse transcriptase-polymerase chain reaction for diagnosis and serotypic characterization of dengue and chikungunya viruses in clinical samples', Diagnostic Microbiology and Infectious Disease, vol. 71, no. 2, pp. 118-125. https://doi.org/10.1016/j.diagmicrobio.2011.06.020

Utility of multiplex reverse transcriptase-polymerase chain reaction for diagnosis and serotypic characterization of dengue and chikungunya viruses in clinical samples. / Mishra, Baijayantimala; Sharma, Mirnalini; Pujhari, Sujit Kumar et al.
In: Diagnostic Microbiology and Infectious Disease, Vol. 71, No. 2, 10.2011, p. 118-125.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Utility of multiplex reverse transcriptase-polymerase chain reaction for diagnosis and serotypic characterization of dengue and chikungunya viruses in clinical samples

AU - Mishra, Baijayantimala

AU - Sharma, Mirnalini

AU - Pujhari, Sujit Kumar

AU - Ratho, Radha Kanta

AU - Gopal, DVR V.R.S.

AU - Kumar, CVM Naresh

AU - Sarangi, Gita

AU - Chayani, Nirupama

AU - Varma, Subhash C.

N1 - Funding Information: The authors acknowledge the financial grant received from the Department of Science and Technology (DST), New Delhi, India, for this work.

PY - 2011/10

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N2 - The reemergence of chikungunya virus (CHIKV) has compounded the already existing dengue problem because of clinical similarities and common vector, demanding the need for a rapid and specific diagnosis. Thus, dengue chikungunya multiplex reverse transcriptase-polymerase chain reaction (DCmRT-PCR) was developed and validated for simultaneous detection of dengue and chikungunya viral infections and its utility in virus serotyping. Blood samples from 97 suspected dengue and chikungunya cases and 10 healthy controls were subjected to dengue and chikungunya conventional RT-PCR and DCmRT-PCR. Thirty-one of 97 samples were positive for dengue or chikungunya viral RNA by RT-PCR and DCmRT-PCR with 100% concordance. DCmRT-PCR products were cycle sequenced. Seven dengue virus strains were clustered within genotype III of DENV-3 and 4 within genotype III of DENV-1, whereas chikungunya sequences were clustered within the Central/East African genotype. DCmRT-PCR was found to be a potential rapid test for simultaneous detection of dengue and CHIKV in clinical samples along with dengue serotyping.

AB - The reemergence of chikungunya virus (CHIKV) has compounded the already existing dengue problem because of clinical similarities and common vector, demanding the need for a rapid and specific diagnosis. Thus, dengue chikungunya multiplex reverse transcriptase-polymerase chain reaction (DCmRT-PCR) was developed and validated for simultaneous detection of dengue and chikungunya viral infections and its utility in virus serotyping. Blood samples from 97 suspected dengue and chikungunya cases and 10 healthy controls were subjected to dengue and chikungunya conventional RT-PCR and DCmRT-PCR. Thirty-one of 97 samples were positive for dengue or chikungunya viral RNA by RT-PCR and DCmRT-PCR with 100% concordance. DCmRT-PCR products were cycle sequenced. Seven dengue virus strains were clustered within genotype III of DENV-3 and 4 within genotype III of DENV-1, whereas chikungunya sequences were clustered within the Central/East African genotype. DCmRT-PCR was found to be a potential rapid test for simultaneous detection of dengue and CHIKV in clinical samples along with dengue serotyping.

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U2 - 10.1016/j.diagmicrobio.2011.06.020

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SN - 0732-8893

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Utility of multiplex reverse transcriptase-polymerase chain reaction for diagnosis and serotypic characterization of dengue and chikungunya viruses in clinical samples

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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