Validation of a commercially available anti-REDD1 antibody using RNA interference and REDD1-/- mouse embryonic fibroblasts

Biochemistry, Genetics and Molecular Biology

• RNAI 100%
• Protein Expression 50%
• Growth Factor 50%
• Western Blot 50%
• Endoplasmic Reticulum Stress 50%
• P53 50%
• Cell Growth 50%
• DNA Damage 50%
• Mammalian Target of Rapamycin Complex 1 50%
• HEK 293 Cells 50%
• Polyclonal Antibody 50%
• Cell Stress 50%
• Antibody Specificity 50%

Immunology and Microbiology

• RNAI 100%
• Western Blot 50%
• Growth Factor 50%
• Cell Line 50%
• Cell Growth 50%
• P53 50%
• Polyclonal Antibody 50%
• Antibody Specificity 50%
• Protein Expression 50%
• Endoplasmic Reticulum Stress 50%
• DNA Damage 50%
• Mammalian Target of Rapamycin Complex 1 50%
• Cell Stress 50%

Keyphrases

• Commercially Available 100%
• Mouse Embryonic Fibroblasts 100%
• RNA Interference 100%
• REDD+ 100%
• Thapsigargin 18%
• Mammalian Target of Rapamycin (mTOR) 9%
• Protein Expression 9%
• Cell Growth 9%
• Inducer 9%
• Tumor Cells 9%
• Endoplasmic Reticulum Stress (ER stress) 9%
• Growth Factor Signaling 9%
• Protein Phosphatase 2A (PP2A) 9%
• DNA Damage 9%
• HEK293 Cells 9%
• Western Blotting 9%
• Hypoxia 9%
• Endogenous Expression 9%
• Cellular Stress 9%
• Polyclonal Antibody 9%
• Antibody Specificity 9%
• Knockout Cells 9%
• Tumor Suppression 9%
• Hypoxia-inducible factor-1α (HIF-1α) 9%
• Uphold 9%
• Growth Pathway 9%
• Stress Factors 9%
• Convergence Point 9%
• Nutrient Deprivation 9%
• Transcriptional Targets 9%