TY - JOUR
T1 - Valvular and Mural Endocardiosis in Aging Zebrafish (Danio rerio)
AU - Cooper, T. K.
AU - Spitsbergen, J. M.
N1 - Funding Information:
The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Parts of this work were funded by the US Public Health Service, National Institutes of Environmental Health Sciences (grants R01ES011587, R21ES013124, P30ESO3850, and P30ES00210), the National Center for Research Resources (grant 3P40RR12546 and its supplement 03S1), and the US Army (contract DAMD 17-91Z1043). The Zebrafish International Resource Center is supported by the National Institutes of Health Office of Research Infrastructure Programs.
Publisher Copyright:
© 2015, © The Author(s) 2015.
PY - 2016/3/1
Y1 - 2016/3/1
N2 - Endocardiosis or myxomatous degeneration of the cardiac valves is a well-described age-related change in humans and dogs. Lesions consist of polypoid nodular proliferations of loose extracellular matrix and valvular interstitial cells, most commonly affecting the mitral valve. This entity has not been previously described in fish. Herein we report the appearance, location, and occurrence of valvular and mural endocardiosis in a retrospective survey of aging laboratory zebrafish. Endocardiosis was present in 59 of 777 fish (7.59%), most commonly affecting the sinoatrial (34 fish; 57.6%) and atrioventricular (33 fish; 55.9%) valves. Lesions were more common in fish raised in recirculating water systems and fed commercial diets (52/230 fish; 22.6%) versus flow-through systems with fish fed semi-purified diets (4/234; 1.71%). Lesions were overrepresented in fish heterozygous for a mutant smoothened allele (34/61 fish, 55.7% vs 17/168, 10.1% wild type). There was no association between endocardiosis and intestinal carcinoids. Valvular endocardiosis is a significant age- and husbandry-related background finding in zebrafish and should be considered in the design and interpretation of research studies.
AB - Endocardiosis or myxomatous degeneration of the cardiac valves is a well-described age-related change in humans and dogs. Lesions consist of polypoid nodular proliferations of loose extracellular matrix and valvular interstitial cells, most commonly affecting the mitral valve. This entity has not been previously described in fish. Herein we report the appearance, location, and occurrence of valvular and mural endocardiosis in a retrospective survey of aging laboratory zebrafish. Endocardiosis was present in 59 of 777 fish (7.59%), most commonly affecting the sinoatrial (34 fish; 57.6%) and atrioventricular (33 fish; 55.9%) valves. Lesions were more common in fish raised in recirculating water systems and fed commercial diets (52/230 fish; 22.6%) versus flow-through systems with fish fed semi-purified diets (4/234; 1.71%). Lesions were overrepresented in fish heterozygous for a mutant smoothened allele (34/61 fish, 55.7% vs 17/168, 10.1% wild type). There was no association between endocardiosis and intestinal carcinoids. Valvular endocardiosis is a significant age- and husbandry-related background finding in zebrafish and should be considered in the design and interpretation of research studies.
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U2 - 10.1177/0300985815594853
DO - 10.1177/0300985815594853
M3 - Article
C2 - 26169384
AN - SCOPUS:84960154830
SN - 0300-9858
VL - 53
SP - 504
EP - 509
JO - Veterinary Pathology
JF - Veterinary Pathology
IS - 2
ER -