TY - JOUR
T1 - Variability in the cadherin gene in an Ostrinia nubilalis strain selected for Cry1Ab resistance
AU - Bel, Yolanda
AU - Siqueira, Herbert A.A.
AU - Siegfried, Blair D.
AU - Ferré, Juan
AU - Escriche, Baltasar
N1 - Funding Information:
Support for this project at the University of Valencia was provided by the Spanish Ministerio de Educación y Ciencia (Project Ref. No. AGL2006-11914) and by European FEDER funds. Support to this work at the University of Nebraska was provided by T. Spencer who was responsible for lab rearing and selection of the Europe-R Strain.
PY - 2009/3
Y1 - 2009/3
N2 - Transgenic corn expressing Cry1Ab (a Bacillus thuringiensis toxin) is highly effective in the control of Ostrinia nubilalis. For its toxic action, Cry1Ab has to bind to specific insect midgut proteins. To date, in three Lepidoptera species resistance to a Cry1A toxin has been conferred by mutations in cadherin, a protein of the Lepidoptera midgut membrane. The implication of cadherin in the resistance of an Ostrinia nubilalis colony (Europe-R) selected with Bacillus thuringiensis Cry1Ab protoxin was investigated. Several major mutations in the cadherin (cdh) gene were found, which introduced premature termination codons and/or large deletions (ranging from 1383 to 1701 bp). The contribution of these major mutations to the resistance was analyzed in resistant individuals that survived exposure to a high concentration of Cry1Ab protoxin. The results indicated that the presence of major mutations was drastically reduced in individuals that survived exposure. Previous inheritance experiments with the Europe-R strain indicated the involvement of more than one genetic locus and reduced amounts of the cadherin receptor. The results of the present work support a polygenic inheritance of resistance in the Europe-R strain, in which mutations in the cdh gene would contribute to resistance by means of an additive effect.
AB - Transgenic corn expressing Cry1Ab (a Bacillus thuringiensis toxin) is highly effective in the control of Ostrinia nubilalis. For its toxic action, Cry1Ab has to bind to specific insect midgut proteins. To date, in three Lepidoptera species resistance to a Cry1A toxin has been conferred by mutations in cadherin, a protein of the Lepidoptera midgut membrane. The implication of cadherin in the resistance of an Ostrinia nubilalis colony (Europe-R) selected with Bacillus thuringiensis Cry1Ab protoxin was investigated. Several major mutations in the cadherin (cdh) gene were found, which introduced premature termination codons and/or large deletions (ranging from 1383 to 1701 bp). The contribution of these major mutations to the resistance was analyzed in resistant individuals that survived exposure to a high concentration of Cry1Ab protoxin. The results indicated that the presence of major mutations was drastically reduced in individuals that survived exposure. Previous inheritance experiments with the Europe-R strain indicated the involvement of more than one genetic locus and reduced amounts of the cadherin receptor. The results of the present work support a polygenic inheritance of resistance in the Europe-R strain, in which mutations in the cdh gene would contribute to resistance by means of an additive effect.
UR - http://www.scopus.com/inward/record.url?scp=61349144246&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=61349144246&partnerID=8YFLogxK
U2 - 10.1016/j.ibmb.2008.11.005
DO - 10.1016/j.ibmb.2008.11.005
M3 - Article
C2 - 19114103
AN - SCOPUS:61349144246
SN - 0965-1748
VL - 39
SP - 218
EP - 223
JO - Insect Biochemistry and Molecular Biology
JF - Insect Biochemistry and Molecular Biology
IS - 3
ER -