Variable number of tandem repeats of 9 Plasmodium vivax genes among Southeast Asian isolates

Bo Wang; Myat Htut Nyunt; Seung Gyu Yun; Feng Lu; Yang Cheng; Jin Hee Han; Kwon Soo Ha; Won Sun Park; Seok Ho Hong; Chae Seung Lim; Jun Cao; Jetsumon Sattabongkot; Myat Phone Kyaw; Liwang Cui; Eun Taek Han

doi:10.1016/j.actatropica.2017.01.013

Variable number of tandem repeats of 9 Plasmodium vivax genes among Southeast Asian isolates

Bo Wang, Myat Htut Nyunt, Seung Gyu Yun, Feng Lu, Yang Cheng, Jin Hee Han, Kwon Soo Ha, Won Sun Park, Seok Ho Hong, Chae Seung Lim, Jun Cao, Jetsumon Sattabongkot, Myat Phone Kyaw, Liwang Cui, Eun Taek Han

Entomology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The variable number of tandem repeats (VNTRs) provides valuable information about both the functional and evolutionary aspects of genetic diversity. Comparative analysis of 3 Plasmodium falciparum genomes has shown that more than 9% of its open reading frames (ORFs) harbor VNTRs. Although microsatellites and VNTR genes of P. vivax were reported, the VNTR polymorphism of genes has not been examined widely. In this study, 230 P. vivax genes were analyzed for VNTRs by SERV, and 33 kinds of TR deletions or insertions from 29 P. vivax genes (12.6%) were found. Of these, 9 VNTR fragments from 8 P. vivax genes were used for PCR amplification and sequence analysis to examine the genetic diversity among 134 isolates from four Southeast Asian countries (China, Republic of Korea, Thailand, and Myanmar) with different malaria endemicity. We confirmed the existence of extensive polymorphism of VNTR fragments in field isolates. This detection provides several suitable markers for analysis of the molecular epidemiology of P. vivax field isolates.

Original language	English (US)
Pages (from-to)	161-168
Number of pages	8
Journal	Acta Tropica
Volume	170
DOIs	https://doi.org/10.1016/j.actatropica.2017.01.013
State	Published - Jun 1 2017

All Science Journal Classification (ASJC) codes

Parasitology
Infectious Diseases

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10.1016/j.actatropica.2017.01.013

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title = "Variable number of tandem repeats of 9 Plasmodium vivax genes among Southeast Asian isolates",

abstract = "The variable number of tandem repeats (VNTRs) provides valuable information about both the functional and evolutionary aspects of genetic diversity. Comparative analysis of 3 Plasmodium falciparum genomes has shown that more than 9% of its open reading frames (ORFs) harbor VNTRs. Although microsatellites and VNTR genes of P. vivax were reported, the VNTR polymorphism of genes has not been examined widely. In this study, 230 P. vivax genes were analyzed for VNTRs by SERV, and 33 kinds of TR deletions or insertions from 29 P. vivax genes (12.6%) were found. Of these, 9 VNTR fragments from 8 P. vivax genes were used for PCR amplification and sequence analysis to examine the genetic diversity among 134 isolates from four Southeast Asian countries (China, Republic of Korea, Thailand, and Myanmar) with different malaria endemicity. We confirmed the existence of extensive polymorphism of VNTR fragments in field isolates. This detection provides several suitable markers for analysis of the molecular epidemiology of P. vivax field isolates.",

author = "Bo Wang and Nyunt, {Myat Htut} and Yun, {Seung Gyu} and Feng Lu and Yang Cheng and Han, {Jin Hee} and Ha, {Kwon Soo} and Park, {Won Sun} and Hong, {Seok Ho} and Lim, {Chae Seung} and Jun Cao and Jetsumon Sattabongkot and Kyaw, {Myat Phone} and Liwang Cui and Han, {Eun Taek}",

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year = "2017",

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day = "1",

doi = "10.1016/j.actatropica.2017.01.013",

language = "English (US)",

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T1 - Variable number of tandem repeats of 9 Plasmodium vivax genes among Southeast Asian isolates

AU - Wang, Bo

AU - Nyunt, Myat Htut

AU - Yun, Seung Gyu

AU - Lu, Feng

AU - Cheng, Yang

AU - Han, Jin Hee

AU - Ha, Kwon Soo

AU - Park, Won Sun

AU - Hong, Seok Ho

AU - Lim, Chae Seung

AU - Cao, Jun

AU - Sattabongkot, Jetsumon

AU - Kyaw, Myat Phone

AU - Cui, Liwang

AU - Han, Eun Taek

PY - 2017/6/1

Y1 - 2017/6/1

N2 - The variable number of tandem repeats (VNTRs) provides valuable information about both the functional and evolutionary aspects of genetic diversity. Comparative analysis of 3 Plasmodium falciparum genomes has shown that more than 9% of its open reading frames (ORFs) harbor VNTRs. Although microsatellites and VNTR genes of P. vivax were reported, the VNTR polymorphism of genes has not been examined widely. In this study, 230 P. vivax genes were analyzed for VNTRs by SERV, and 33 kinds of TR deletions or insertions from 29 P. vivax genes (12.6%) were found. Of these, 9 VNTR fragments from 8 P. vivax genes were used for PCR amplification and sequence analysis to examine the genetic diversity among 134 isolates from four Southeast Asian countries (China, Republic of Korea, Thailand, and Myanmar) with different malaria endemicity. We confirmed the existence of extensive polymorphism of VNTR fragments in field isolates. This detection provides several suitable markers for analysis of the molecular epidemiology of P. vivax field isolates.

AB - The variable number of tandem repeats (VNTRs) provides valuable information about both the functional and evolutionary aspects of genetic diversity. Comparative analysis of 3 Plasmodium falciparum genomes has shown that more than 9% of its open reading frames (ORFs) harbor VNTRs. Although microsatellites and VNTR genes of P. vivax were reported, the VNTR polymorphism of genes has not been examined widely. In this study, 230 P. vivax genes were analyzed for VNTRs by SERV, and 33 kinds of TR deletions or insertions from 29 P. vivax genes (12.6%) were found. Of these, 9 VNTR fragments from 8 P. vivax genes were used for PCR amplification and sequence analysis to examine the genetic diversity among 134 isolates from four Southeast Asian countries (China, Republic of Korea, Thailand, and Myanmar) with different malaria endemicity. We confirmed the existence of extensive polymorphism of VNTR fragments in field isolates. This detection provides several suitable markers for analysis of the molecular epidemiology of P. vivax field isolates.

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JO - Acta Tropica

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ER -

Variable number of tandem repeats of 9 Plasmodium vivax genes among Southeast Asian isolates

Abstract

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