TY - JOUR
T1 - Variation in the ribosomal DNA internal transcribed spacer of a diverse collection of ectomycorrhizal fungi
AU - Farmer, David J.
AU - Sylvia, David M.
N1 - Funding Information:
Pure culture isolates were provided by Jane E. Smith, Forestry Sciences Laboratory, USDA Forest Service, Pacific Northwest Research Station, Corvallis, OR, D. H. Marx, Institute for Mycorrhizal Research and Development, USDA Forest Service, Southeastern Forest Experiment Station, Athens, GA, and Peter Jeffries, University of Kent, Canterbury, U.K. We thank Frank Martin for guidance and laboratory support with molecular techniques, Brian Bowen and Gerald Benny for their assistance with the PHYLIP programs, and Monique Gardes and Andy Ogram for review comments. This work was partially supported by A Partnership for Fundamental Research in Forest Biology. Published as Florida Agricultural Experimental Station Journal Series no. R-05164.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1998/7
Y1 - 1998/7
N2 - Amplification of the internal transcribed spacer (ITS) of ribosomal RNA genes using PCR and subsequent RFLP analysis offers promise for improved species-level identification of ectomycorrhizal fungi. Intrageneric homogeneity and intraspecific heterogeneity of these characters are, however, frequent. A diverse group of ectomycorrhizal fungi with worldwide distribution was characterized by ITS-RFLP analysis. DNA of 64 identified and five unknown ectomycorrhizal fungi was extracted and their ITS regions were selectively amplified with ITS1-F and ITS4 ribosomal RNA gene primers. Each PCR product was digested with seven restriction enzymes. The resulting restriction fragments were compared by distance matrix analysis. Restriction enzyme analysis of amplified fragments was sufficient for distinguishing all but two closely related species. Intraspecific variation was observed in Cenococcum geophilum and Pisolithus arhizus, providing further evidence that these taxa represent species complexes.
AB - Amplification of the internal transcribed spacer (ITS) of ribosomal RNA genes using PCR and subsequent RFLP analysis offers promise for improved species-level identification of ectomycorrhizal fungi. Intrageneric homogeneity and intraspecific heterogeneity of these characters are, however, frequent. A diverse group of ectomycorrhizal fungi with worldwide distribution was characterized by ITS-RFLP analysis. DNA of 64 identified and five unknown ectomycorrhizal fungi was extracted and their ITS regions were selectively amplified with ITS1-F and ITS4 ribosomal RNA gene primers. Each PCR product was digested with seven restriction enzymes. The resulting restriction fragments were compared by distance matrix analysis. Restriction enzyme analysis of amplified fragments was sufficient for distinguishing all but two closely related species. Intraspecific variation was observed in Cenococcum geophilum and Pisolithus arhizus, providing further evidence that these taxa represent species complexes.
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U2 - 10.1017/S0953756297005601
DO - 10.1017/S0953756297005601
M3 - Article
AN - SCOPUS:0031847314
SN - 0953-7562
VL - 102
SP - 859
EP - 865
JO - Mycological Research
JF - Mycological Research
IS - 7
ER -