TY - JOUR
T1 - Variation of acharan sulfate and monosaccharide composition and analysis of neutral N-glycans in African giant snail (Achatina fulica)
AU - Park, Youmie
AU - Zhang, Zhenqing
AU - Laremore, Tatiana N.
AU - Li, Boyangzi
AU - Sim, Joon Soo
AU - Im, A. Rang
AU - Ahn, Mi Young
AU - Kim, Yeong Shik
AU - Linhardt, Robert J.
N1 - Funding Information:
Acknowledgements Authors thank Professor Toshihiko Toida of Chiba University for assistance in NMR interpretation. The authors also acknowledge funding from the U.S. National Institutes of Health (grants GM38060 and HL62244) to Robert J. Linhardt, and from the KOSEF grant RO1-1999-2-209-010-5 to YSK. This project was partially supported by the grant from National Institute of Agricultural Biotechnology, Suwon, Republic of Korea.
PY - 2008/12
Y1 - 2008/12
N2 - Acharan sulfate content from African giant snail (Achatina fulica) was compared in eggs and snails of different ages. Acharan sulfate was not found in egg. Acharan sulfate disaccharide →4)-α-d-GlcNpAc (1→4)-α-l-IdoAp2S(1→, analyzed by SAX (strong-anion exchange)-HPLC was observed soon after hatching and increases as the snails grow. Monosaccharide compositional analysis showed that mole % of glucosamine, a major monosaccharide of acharan sulfate, increased with age while mole % of galactose decreased with age. These results suggest that galactans represent a major energy source during development, while acharan sulfate appearing immediately after hatching, is essential for the snail growth. The structures of neutral N-glycans released from eggs by peptide N-glycosidase F (PNGase F), were next elucidated using ESI-MS/MS, MALDI-MS/MS, enzyme digestion, and monosaccharide composition analysis. Three types of neutral N-glycan structures were observed, truncated (Hex2-4-HexNAc2), high mannose (Hex5-9-HexNAc2), and complex (Hex3-HexNAc 2-10) types. None showed core fucosylation.
AB - Acharan sulfate content from African giant snail (Achatina fulica) was compared in eggs and snails of different ages. Acharan sulfate was not found in egg. Acharan sulfate disaccharide →4)-α-d-GlcNpAc (1→4)-α-l-IdoAp2S(1→, analyzed by SAX (strong-anion exchange)-HPLC was observed soon after hatching and increases as the snails grow. Monosaccharide compositional analysis showed that mole % of glucosamine, a major monosaccharide of acharan sulfate, increased with age while mole % of galactose decreased with age. These results suggest that galactans represent a major energy source during development, while acharan sulfate appearing immediately after hatching, is essential for the snail growth. The structures of neutral N-glycans released from eggs by peptide N-glycosidase F (PNGase F), were next elucidated using ESI-MS/MS, MALDI-MS/MS, enzyme digestion, and monosaccharide composition analysis. Three types of neutral N-glycan structures were observed, truncated (Hex2-4-HexNAc2), high mannose (Hex5-9-HexNAc2), and complex (Hex3-HexNAc 2-10) types. None showed core fucosylation.
UR - https://www.scopus.com/pages/publications/56949095315
UR - https://www.scopus.com/pages/publications/56949095315#tab=citedBy
U2 - 10.1007/s10719-008-9149-1
DO - 10.1007/s10719-008-9149-1
M3 - Article
C2 - 18670878
AN - SCOPUS:56949095315
SN - 0282-0080
VL - 25
SP - 863
EP - 877
JO - Glycoconjugate Journal
JF - Glycoconjugate Journal
IS - 9
ER -