TY - JOUR
T1 - Vector specificity of Barley Yellow Dwarf Virus (BYDV) transmission
T2 - Identification of potential cellular receptors binding BYDV-MAV in the aphid, Sitobion avenae
AU - Li, Chaoyang
AU - Cox-Foster, Diana
AU - Gray, Stewart M.
AU - Gildow, Frederick
N1 - Funding Information:
This work was supported by Grant MCB-9419807 from the National Science Foundation. We thank William Sackett for technical assistance.
PY - 2001/7/20
Y1 - 2001/7/20
N2 - Two proteins (SaM35 and SaM50) isolated from head tissues of the aphid vector, Sitobion avenae, were identified as potential receptors for barley yellow dwarf virus MAV isolate (Luteoviridae) based on MAV virus overlay assays and immunoblots of urea SDS 2-D gels. An anti-idiotypic antibody (MAV4 anti-ID) that mimics an epitope on MAV virions and competes with MAV in antibody binding assays also bound to SaM50 and SaM35 and to six additional proteins including a GroEL homolog No MAV-binding proteins were detected from the nonvector aphid, Rhopatosiphum maidis, although MAV4 anti-ID did react with four proteins from R. maidis. It is hypothesized that SaM35 and SaM50 may be MAV receptors involved in MAV transmission based on their high affinity for MAV and their unique association with the vector, S. avenae. The additional aphid proteins binding the MAV4 anti-ID may represent less specific virus-binding proteins facilitating transmission through different aphid tissues.
AB - Two proteins (SaM35 and SaM50) isolated from head tissues of the aphid vector, Sitobion avenae, were identified as potential receptors for barley yellow dwarf virus MAV isolate (Luteoviridae) based on MAV virus overlay assays and immunoblots of urea SDS 2-D gels. An anti-idiotypic antibody (MAV4 anti-ID) that mimics an epitope on MAV virions and competes with MAV in antibody binding assays also bound to SaM50 and SaM35 and to six additional proteins including a GroEL homolog No MAV-binding proteins were detected from the nonvector aphid, Rhopatosiphum maidis, although MAV4 anti-ID did react with four proteins from R. maidis. It is hypothesized that SaM35 and SaM50 may be MAV receptors involved in MAV transmission based on their high affinity for MAV and their unique association with the vector, S. avenae. The additional aphid proteins binding the MAV4 anti-ID may represent less specific virus-binding proteins facilitating transmission through different aphid tissues.
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U2 - 10.1006/viro.2001.0929
DO - 10.1006/viro.2001.0929
M3 - Article
C2 - 11448166
AN - SCOPUS:0035919707
SN - 0042-6822
VL - 286
SP - 125
EP - 133
JO - Virology
JF - Virology
IS - 1
ER -