Vesicle Collision Protocols for the Study of Quantum Size and Exocytotic Fraction Released

Soodabeh Majdi, Alex S. Lima, Andrew G. Ewing

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

We review the methods of vesicle impact electrochemical cytometry, intracellular impact electrochemical cytometry, and single cell amperometry and their application to measuring the storage of neurotransmitters in cellular vesicles. We provide protocols to measure vesicle content, the release of catecholamines, and from there the fraction of transmitter released in each exocytosis event. The focus here has been a combination of methods to evaluate factors related to neuronal function at the cellular level and implications in, for example, cognition.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages223-237
Number of pages15
DOIs
StatePublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2565
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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