Vesicular Transmitter Content in Chromaffin Cells Can Be Regulated via Extracellular ATP

Anna Larsson, Soodabeh Majdi, Ricardo Borges, Andrew Ewing

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


The energy carrying molecule adenosine triphosphate (ATP) has been implicated for its role in modulation of chemical signaling for some time. Despite this, the precise effects and mechanisms of action of ATP on secretory cells are not well-known. Here, bovine chromaffin cells have been used as a model system to study the effects of extracellular ATP in combination with the catecholamine transmitter norepinephrine (NE). Both transmitter storage and exocytotic release were quantified using complementary amperometric techniques. Although incubation with NE alone did not cause any changes to either transmitter storage or release, coincubation with NE and ATP resulted in a significant increase that was concentration dependent. To probe the potential mechanisms of action, a slowly hydrolyzable version of ATP, ATP-γ-S, was used either alone or together with NE. The result implicates two different behaviors of ATP acting on both the purinergic autoreceptors and as a source of the energy needed to load chromaffin cell vesicles.

Original languageEnglish (US)
Pages (from-to)4735-4740
Number of pages6
JournalACS Chemical Neuroscience
Issue number11
StatePublished - Nov 20 2019

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Cognitive Neuroscience
  • Cell Biology


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