Viability of human marrow after long-term cryopreservation

Witold Rybka, Karl Mittermeyer, Jack W. Singer, C. Dean Buckner, E. Donnall Thomas

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Human bone marrow specimens cryopreserved with dimethyl sulfoxide (Me2SO) and stored in the vapor phase of liquid nitrogen for 37 to 121 months were evaluated for viability using the granulocyte—macrophage (CFU-C) and burst-forming (BFU-E) assays. Eighteen marrows were from patients with a variety of malignant diseases and 10 were from patients with chronic granulocytic leukemia (CGL). Twenty-two of the twenty-eight stored marrows showed in vitro colony growth by at least one of the assays. Four of the marrows from patients with malignant diseases and two from patients with CGL showed no growth. All marrows showing no colony growth had been stored at least 8.5 years, although colony growth was seen in marrows stored for up to 10 years.

Original languageEnglish (US)
Pages (from-to)424-428
Number of pages5
Issue number5
StatePublished - 1980

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)


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