Viability of human marrow after long-term cryopreservation

Witold Rybka; Karl Mittermeyer; Jack W. Singer; C. Dean Buckner; E. Donnall Thomas

doi:10.1016/0011-2240(80)90051-6

Viability of human marrow after long-term cryopreservation

Witold Rybka
, Karl Mittermeyer
, Jack W. Singer
, C. Dean Buckner
, E. Donnall Thomas

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Human bone marrow specimens cryopreserved with dimethyl sulfoxide (Me₂SO) and stored in the vapor phase of liquid nitrogen for 37 to 121 months were evaluated for viability using the granulocyte—macrophage (CFU-C) and burst-forming (BFU-E) assays. Eighteen marrows were from patients with a variety of malignant diseases and 10 were from patients with chronic granulocytic leukemia (CGL). Twenty-two of the twenty-eight stored marrows showed in vitro colony growth by at least one of the assays. Four of the marrows from patients with malignant diseases and two from patients with CGL showed no growth. All marrows showing no colony growth had been stored at least 8.5 years, although colony growth was seen in marrows stored for up to 10 years.

Original language	English (US)
Pages (from-to)	424-428
Number of pages	5
Journal	Cryobiology
Volume	17
Issue number	5
DOIs	https://doi.org/10.1016/0011-2240(80)90051-6
State	Published - 1980

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences

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10.1016/0011-2240(80)90051-6

Cite this

@article{bd87fad222bb495ebeb6daaf3e78291c,

title = "Viability of human marrow after long-term cryopreservation",

abstract = "Human bone marrow specimens cryopreserved with dimethyl sulfoxide (Me2SO) and stored in the vapor phase of liquid nitrogen for 37 to 121 months were evaluated for viability using the granulocyte—macrophage (CFU-C) and burst-forming (BFU-E) assays. Eighteen marrows were from patients with a variety of malignant diseases and 10 were from patients with chronic granulocytic leukemia (CGL). Twenty-two of the twenty-eight stored marrows showed in vitro colony growth by at least one of the assays. Four of the marrows from patients with malignant diseases and two from patients with CGL showed no growth. All marrows showing no colony growth had been stored at least 8.5 years, although colony growth was seen in marrows stored for up to 10 years.",

author = "Witold Rybka and Karl Mittermeyer and Singer, \{Jack W.\} and Buckner, \{C. Dean\} and Thomas, \{E. Donnall\}",

note = "Funding Information: Received December 4, 1979; accepted June 3, 1980. I This investigation was supported by Grant CA 18029, awarded by the National Cancer Institute, DHEW, and Grant CI-52K from the American Cancer Society. 2 Dr. Rybka is the recipient of a fellowship from the R. Samuel McLaughlin Foundation, Toronto, Ontario, Canada. 3 Dr. Mittermeyer was supported in part by an award from the Max Kade Foundation, New York. 4 Dr. Thomas is a recipient of a Research Career Award AI 02425 from the National Institute of Allergy and Infectious Diseases.",

year = "1980",

doi = "10.1016/0011-2240(80)90051-6",

language = "English (US)",

volume = "17",

pages = "424--428",

journal = "Cryobiology",

issn = "0011-2240",

publisher = "Academic Press Inc.",

number = "5",

}

TY - JOUR

T1 - Viability of human marrow after long-term cryopreservation

AU - Rybka, Witold

AU - Mittermeyer, Karl

AU - Singer, Jack W.

AU - Buckner, C. Dean

AU - Thomas, E. Donnall

N1 - Funding Information: Received December 4, 1979; accepted June 3, 1980. I This investigation was supported by Grant CA 18029, awarded by the National Cancer Institute, DHEW, and Grant CI-52K from the American Cancer Society. 2 Dr. Rybka is the recipient of a fellowship from the R. Samuel McLaughlin Foundation, Toronto, Ontario, Canada. 3 Dr. Mittermeyer was supported in part by an award from the Max Kade Foundation, New York. 4 Dr. Thomas is a recipient of a Research Career Award AI 02425 from the National Institute of Allergy and Infectious Diseases.

PY - 1980

Y1 - 1980

N2 - Human bone marrow specimens cryopreserved with dimethyl sulfoxide (Me2SO) and stored in the vapor phase of liquid nitrogen for 37 to 121 months were evaluated for viability using the granulocyte—macrophage (CFU-C) and burst-forming (BFU-E) assays. Eighteen marrows were from patients with a variety of malignant diseases and 10 were from patients with chronic granulocytic leukemia (CGL). Twenty-two of the twenty-eight stored marrows showed in vitro colony growth by at least one of the assays. Four of the marrows from patients with malignant diseases and two from patients with CGL showed no growth. All marrows showing no colony growth had been stored at least 8.5 years, although colony growth was seen in marrows stored for up to 10 years.

AB - Human bone marrow specimens cryopreserved with dimethyl sulfoxide (Me2SO) and stored in the vapor phase of liquid nitrogen for 37 to 121 months were evaluated for viability using the granulocyte—macrophage (CFU-C) and burst-forming (BFU-E) assays. Eighteen marrows were from patients with a variety of malignant diseases and 10 were from patients with chronic granulocytic leukemia (CGL). Twenty-two of the twenty-eight stored marrows showed in vitro colony growth by at least one of the assays. Four of the marrows from patients with malignant diseases and two from patients with CGL showed no growth. All marrows showing no colony growth had been stored at least 8.5 years, although colony growth was seen in marrows stored for up to 10 years.

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UR - https://www.scopus.com/pages/publications/0019206206#tab=citedBy

U2 - 10.1016/0011-2240(80)90051-6

DO - 10.1016/0011-2240(80)90051-6

M3 - Article

C2 - 6934065

AN - SCOPUS:0019206206

SN - 0011-2240

VL - 17

SP - 424

EP - 428

JO - Cryobiology

JF - Cryobiology

IS - 5

ER -

Viability of human marrow after long-term cryopreservation

Abstract

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All Science Journal Classification (ASJC) codes

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