TY - GEN
T1 - Viable circulating tumor cell enrichment by flexible micro spring array
AU - Harouaka, Ramdane A.
AU - Zhou, Ming Da
AU - Yeh, Tim Y.
AU - Khan, Waleed J.
AU - Allerton, Jeffery
AU - Zheng, Si Yang
PY - 2012
Y1 - 2012
N2 - We demonstrated a high throughput versatile platform capable of isolating circulating tumor cells (CTCs) from clinically relevant volumes of blood while preserving their viability and ability to proliferate. The enrichment is based on the fact that CTCs are larger compared with normal blood cells. The incorporated system allows size-based separation of CTCs at the micro-scale, while taking advantage of a high throughput and rapid processing speed. Testing results of model systems using cell lines show that this device can enrich CTCs from 7.5 mL of whole blood samples with 90% capture efficiency, higher than 104 enrichment, and better than 80% viability in approximately ten minutes without any incidence of clogging.
AB - We demonstrated a high throughput versatile platform capable of isolating circulating tumor cells (CTCs) from clinically relevant volumes of blood while preserving their viability and ability to proliferate. The enrichment is based on the fact that CTCs are larger compared with normal blood cells. The incorporated system allows size-based separation of CTCs at the micro-scale, while taking advantage of a high throughput and rapid processing speed. Testing results of model systems using cell lines show that this device can enrich CTCs from 7.5 mL of whole blood samples with 90% capture efficiency, higher than 104 enrichment, and better than 80% viability in approximately ten minutes without any incidence of clogging.
UR - http://www.scopus.com/inward/record.url?scp=84870802939&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84870802939&partnerID=8YFLogxK
U2 - 10.1109/EMBC.2012.6347427
DO - 10.1109/EMBC.2012.6347427
M3 - Conference contribution
C2 - 23367362
AN - SCOPUS:84870802939
SN - 9781424441198
T3 - Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
SP - 6269
EP - 6272
BT - 2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2012
T2 - 34th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2012
Y2 - 28 August 2012 through 1 September 2012
ER -