Viable circulating tumor cell enrichment by flexible micro spring array.

Ramdane A. Harouaka; Ming Da Zhou; Tim Y. Yeh; Waleed J. Khan; Jeffery Allerton; Si Yang Zheng

Viable circulating tumor cell enrichment by flexible micro spring array.

Ramdane A. Harouaka, Ming Da Zhou, Tim Y. Yeh, Waleed J. Khan, Jeffery Allerton, Si Yang Zheng

Research output: Contribution to journal › Article › peer-review

Abstract

We demonstrated a high throughput versatile platform capable of isolating circulating tumor cells (CTCs) from clinically relevant volumes of blood while preserving their viability and ability to proliferate. The enrichment is based on the fact that CTCs are larger compared with normal blood cells. The incorporated system allows size-based separation of CTCs at the micro-scale, while taking advantage of a high throughput and rapid processing speed. Testing results of model systems using cell lines show that this device can enrich CTCs from 7.5 mL of whole blood samples with 90% capture efficiency, higher than 10(4) enrichment, and better than 80% viability in approximately ten minutes without any incidence of clogging.

Original language	English (US)
Pages (from-to)	6269-6272
Number of pages	4
Journal	Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference
State	Published - 2012

All Science Journal Classification (ASJC) codes

Computer Vision and Pattern Recognition
Signal Processing
Biomedical Engineering
Health Informatics

Cite this

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title = "Viable circulating tumor cell enrichment by flexible micro spring array.",

abstract = "We demonstrated a high throughput versatile platform capable of isolating circulating tumor cells (CTCs) from clinically relevant volumes of blood while preserving their viability and ability to proliferate. The enrichment is based on the fact that CTCs are larger compared with normal blood cells. The incorporated system allows size-based separation of CTCs at the micro-scale, while taking advantage of a high throughput and rapid processing speed. Testing results of model systems using cell lines show that this device can enrich CTCs from 7.5 mL of whole blood samples with 90% capture efficiency, higher than 10(4) enrichment, and better than 80% viability in approximately ten minutes without any incidence of clogging.",

author = "Harouaka, {Ramdane A.} and Zhou, {Ming Da} and Yeh, {Tim Y.} and Khan, {Waleed J.} and Jeffery Allerton and Zheng, {Si Yang}",

year = "2012",

language = "English (US)",

pages = "6269--6272",

journal = "Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference",

issn = "1557-170X",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

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Viable circulating tumor cell enrichment by flexible micro spring array. / Harouaka, Ramdane A.; Zhou, Ming Da; Yeh, Tim Y. et al.
In: Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference, 2012, p. 6269-6272.

Research output: Contribution to journal › Article › peer-review

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T1 - Viable circulating tumor cell enrichment by flexible micro spring array.

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AU - Zhou, Ming Da

AU - Yeh, Tim Y.

AU - Khan, Waleed J.

AU - Allerton, Jeffery

AU - Zheng, Si Yang

PY - 2012

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AB - We demonstrated a high throughput versatile platform capable of isolating circulating tumor cells (CTCs) from clinically relevant volumes of blood while preserving their viability and ability to proliferate. The enrichment is based on the fact that CTCs are larger compared with normal blood cells. The incorporated system allows size-based separation of CTCs at the micro-scale, while taking advantage of a high throughput and rapid processing speed. Testing results of model systems using cell lines show that this device can enrich CTCs from 7.5 mL of whole blood samples with 90% capture efficiency, higher than 10(4) enrichment, and better than 80% viability in approximately ten minutes without any incidence of clogging.

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JO - Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference

JF - Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference

ER -

Viable circulating tumor cell enrichment by flexible micro spring array.

Abstract

All Science Journal Classification (ASJC) codes

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