TY - JOUR
T1 - Virus Assembly Pathways
T2 - Straying Away but Not Too Far
AU - Bond, Kevin
AU - Tsvetkova, Irina B.
AU - Wang, Joseph Che Yen
AU - Jarrold, Martin F.
AU - Dragnea, Bogdan
N1 - Funding Information:
The work was supported by the Army Research Office, under award W911NF-17-1-0329, the National Science Foundation, under award CBET 1803440, and by the NIH under award number 1RO1AI118933. The authors gratefully acknowledge Indiana University at Bloomington for access to the Electron Microscopy Center and the Nanoscience Characterization Facility and the Pennsylvania State University College of Medicine for access to the Thermo Scientific Titan Krios.
Funding Information:
The work was supported by the Army Research Office, under award W911NF‐17‐1‐0329, the National Science Foundation, under award CBET 1803440, and by the NIH under award number 1RO1AI118933. The authors gratefully acknowledge Indiana University at Bloomington for access to the Electron Microscopy Center and the Nanoscience Characterization Facility and the Pennsylvania State University College of Medicine for access to the Thermo Scientific Titan Krios.
Publisher Copyright:
© 2020 Wiley-VCH GmbH
PY - 2020/12/22
Y1 - 2020/12/22
N2 - Non-enveloped RNA viruses pervade all domains of life. In a cell, they co-assemble from viral RNA and capsid proteins. Virus-like particles can form in vitro where virtually any non-cognate polyanionic cargo can be packaged. How only viral RNA gets selected for packaging in vivo, in presence of myriad other polyanionic species, has been a puzzle. Through a combination of charge detection mass spectrometry and cryo-electron microscopy, it is determined that co-assembling brome mosaic virus (BMV) coat proteins and nucleic acid oligomers results in capsid structures and stoichiometries that differ from the icosahedral virion. These previously unknown shell structures are strained and less stable than the native one. However, they contain large native structure fragments that can be recycled to form BMV virions, should a viral genome become available. The existence of such structures suggest the possibility of a previously unknown regulatory pathway for the packaging process inside cells.
AB - Non-enveloped RNA viruses pervade all domains of life. In a cell, they co-assemble from viral RNA and capsid proteins. Virus-like particles can form in vitro where virtually any non-cognate polyanionic cargo can be packaged. How only viral RNA gets selected for packaging in vivo, in presence of myriad other polyanionic species, has been a puzzle. Through a combination of charge detection mass spectrometry and cryo-electron microscopy, it is determined that co-assembling brome mosaic virus (BMV) coat proteins and nucleic acid oligomers results in capsid structures and stoichiometries that differ from the icosahedral virion. These previously unknown shell structures are strained and less stable than the native one. However, they contain large native structure fragments that can be recycled to form BMV virions, should a viral genome become available. The existence of such structures suggest the possibility of a previously unknown regulatory pathway for the packaging process inside cells.
UR - http://www.scopus.com/inward/record.url?scp=85096669640&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85096669640&partnerID=8YFLogxK
U2 - 10.1002/smll.202004475
DO - 10.1002/smll.202004475
M3 - Article
C2 - 33241653
AN - SCOPUS:85096669640
SN - 1613-6810
VL - 16
JO - Small
JF - Small
IS - 51
M1 - 2004475
ER -