TY - JOUR
T1 - Visualizing and Manipulating Biological Processes by Using HaloTag and SNAP-Tag Technologies
AU - Hoelzel, Conner A.
AU - Zhang, Xin
N1 - Funding Information:
This work was supported by the Burroughs Wellcome Fund Career Award at the Scientific Interface (X.Z.), Paul Berg Early Career Professorship (X.Z.), Lloyd and Dottie Huck Early Career Award (X.Z.), Sloan Research Fellowship (X.Z.), Pew Biomedical Scholars Program (X.Z.) and National Institute of Health R35 GM133484 (X.Z.).
Funding Information:
This work was supported by the Burroughs Wellcome Fund Career Award at the Scientific Interface (X.Z.), Paul Berg Early Career Professorship (X.Z.), Lloyd and Dottie Huck Early Career Award (X.Z.), Sloan Research Fellowship (X.Z.), Pew Biomedical Scholars Program (X.Z.) and National Institute of Health R35 GM133484 (X.Z.).
Funding Information:
. Prof. Xin Zhang is the holder of the Paul Berg Early Career Professorship and an Assistant Professor of Chemistry and of Biochemistry and Molecular Biology at Penn State. Zhang's independent work at Penn State has received multiple awards, including NSF CAREER Award, NIGMS MIRA, Pew Scholar in the Biomedical Sciences, Sloan Research Fellowship in Chemistry, the Lloyd and Dottie Huck Early Career Award, the Burroughs Wellcome Fund Career Award at the Scientific Interface
Publisher Copyright:
© 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2020/7/16
Y1 - 2020/7/16
N2 - Visualizing and manipulating the behavior of proteins is crucial to understanding the physiology of the cell. Methods of biorthogonal protein labeling are important tools to attain this goal. In this review, we discuss advances in probe technology specific for self-labeling protein tags, focusing mainly on the application of HaloTag and SNAP-tag systems. We describe the latest developments in small-molecule probes that enable fluorogenic (no wash) imaging and super-resolution fluorescence microscopy. In addition, we cover several methodologies that enable the perturbation or manipulation of protein behavior and function towards the control of biological pathways. Thus, current technical advances in the HaloTag and SNAP-tag systems means that they are becoming powerful tools to enable the visualization and manipulation of biological processes, providing invaluable scientific insights that are difficult to obtain by traditional methodologies. As the multiplex of self-labeling protein tag systems continues to be developed and expanded, the utility of these protein tags will allow researchers to address previously inaccessible questions at the forefront of biology.
AB - Visualizing and manipulating the behavior of proteins is crucial to understanding the physiology of the cell. Methods of biorthogonal protein labeling are important tools to attain this goal. In this review, we discuss advances in probe technology specific for self-labeling protein tags, focusing mainly on the application of HaloTag and SNAP-tag systems. We describe the latest developments in small-molecule probes that enable fluorogenic (no wash) imaging and super-resolution fluorescence microscopy. In addition, we cover several methodologies that enable the perturbation or manipulation of protein behavior and function towards the control of biological pathways. Thus, current technical advances in the HaloTag and SNAP-tag systems means that they are becoming powerful tools to enable the visualization and manipulation of biological processes, providing invaluable scientific insights that are difficult to obtain by traditional methodologies. As the multiplex of self-labeling protein tag systems continues to be developed and expanded, the utility of these protein tags will allow researchers to address previously inaccessible questions at the forefront of biology.
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U2 - 10.1002/cbic.202000037
DO - 10.1002/cbic.202000037
M3 - Review article
C2 - 32180315
AN - SCOPUS:85082836891
SN - 1439-4227
VL - 21
SP - 1935
EP - 1946
JO - ChemBioChem
JF - ChemBioChem
IS - 14
ER -