TY - JOUR
T1 - Vitamin A deficiency increases hepatic apolipoprotein A-I mRNA expression in both euthyroid and hypothyroid rats
AU - Zolfaghari, Reza
AU - Ross, A. Catharine
N1 - Funding Information:
This research was supported in part by National Institutes of Health grant DK-46869 and funds from the Howard Heinz Endowment.
PY - 1997/8
Y1 - 1997/8
N2 - We have examined the effects of chronic thyroid hormone deficiency, vitamin A deficiency, and subsequent acute repletion with thyroid hormone (T3) and/or retinoic acid (RA) on the expression of hepatic apolipoprotein (ape) A-I mRNA. Chronically hypothyroid and/or retinoid-deficient male Lewis rats were treated once or three times with T3, all-trans-RA, or both hormones. The hypothyroid state significantly reduced (P < 0.001) and the vitamin A-deficient state significantly elevated (P < 0.0001) the expression of apo A-I mRNA. A single injection of T3 (10 μg/100 gm body weight) significantly elevated apo A-I mRNA in hypothyroid rats (P < 0. 001). A single injection of RA (20 μg/rat) reduced apo A-I mRNA by about 20 to 25% and daily injection of RA for three days reduced apo A-I mRNA abundance further in hypothyroid (P < 0.05), but not euthyroid rats. There was a significant interaction between T3 hormone treatment and vitamin A status of the rats, as well as between RA and T3 treatment and the thyroid status of the rats. These in vivo results may indicate that nuclear retinoid receptors and thyroid hormone receptors interact in the regulation of the apo A-I gene in liver.
AB - We have examined the effects of chronic thyroid hormone deficiency, vitamin A deficiency, and subsequent acute repletion with thyroid hormone (T3) and/or retinoic acid (RA) on the expression of hepatic apolipoprotein (ape) A-I mRNA. Chronically hypothyroid and/or retinoid-deficient male Lewis rats were treated once or three times with T3, all-trans-RA, or both hormones. The hypothyroid state significantly reduced (P < 0.001) and the vitamin A-deficient state significantly elevated (P < 0.0001) the expression of apo A-I mRNA. A single injection of T3 (10 μg/100 gm body weight) significantly elevated apo A-I mRNA in hypothyroid rats (P < 0. 001). A single injection of RA (20 μg/rat) reduced apo A-I mRNA by about 20 to 25% and daily injection of RA for three days reduced apo A-I mRNA abundance further in hypothyroid (P < 0.05), but not euthyroid rats. There was a significant interaction between T3 hormone treatment and vitamin A status of the rats, as well as between RA and T3 treatment and the thyroid status of the rats. These in vivo results may indicate that nuclear retinoid receptors and thyroid hormone receptors interact in the regulation of the apo A-I gene in liver.
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U2 - 10.1016/S0955-2863(97)00055-7
DO - 10.1016/S0955-2863(97)00055-7
M3 - Article
AN - SCOPUS:0030839725
SN - 0955-2863
VL - 8
SP - 451
EP - 455
JO - Journal of Nutritional Biochemistry
JF - Journal of Nutritional Biochemistry
IS - 8
ER -
