Vliianie gomologichnykh peptidov factorov differentsirovki HLDF i PEDF na doimplantatsionnoe razvitie myshei in vitro.

N. I. Sakharova; I. A. Kostanian; T. N. Lepikhova; K. A. Lepikhov; A. M. Malashenko; E. V. Navolotskaia; J. Tombran-Tink; V. M. Lipkin

Vliianie gomologichnykh peptidov factorov differentsirovki HLDF i PEDF na doimplantatsionnoe razvitie myshei in vitro.

Translated title of the contribution: Effect of homologous peptides of differentiation factors HLDF and PEDF on preimplantation development of mice in vitro

N. I. Sakharova, I. A. Kostanian, T. N. Lepikhova, K. A. Lepikhov, A. M. Malashenko, E. V. Navolotskaia, J. Tombran-Tink, V. M. Lipkin

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We studied the effect of synthetic peptides PEDF-6 and HLDF-6 on preimplantation development of mouse embryos in vitro. PEDF-6 peptide corresponds to fragment 351-356 and of pigment epithelium-derived differentiation factor (PEDF), while HLDF-6 peptide corresponds to fragment 84-89 of differentiation factor HLDF isolated from HL-60 cell line. Despite high homology, these peptides had different effects on the early development. PEDF-6 had no effect on the cleavage of 2-4-cell embryos but decelerated blastocyst formation from such embryos and disturbed their structure. In the presence of HLDF-6 the blastomeres divided more actively as compared to the control and a higher number of embryos developed to the blastocyst stage. The effects of both peptides were stage-specific: the affect the embryos at early cleavage stages and, apparently, determine their further development at that moment although do not directly affect formation of the blastocysts.

Translated title of the contribution	Effect of homologous peptides of differentiation factors HLDF and PEDF on preimplantation development of mice in vitro
Original language	Russian
Pages (from-to)	195-200
Number of pages	6
Journal	Ontogenez
Volume	33
Issue number	3
State	Published - 2002

All Science Journal Classification (ASJC) codes

General Medicine

Cite this

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title = "Vliianie gomologichnykh peptidov factorov differentsirovki HLDF i PEDF na doimplantatsionnoe razvitie myshei in vitro.",

abstract = "We studied the effect of synthetic peptides PEDF-6 and HLDF-6 on preimplantation development of mouse embryos in vitro. PEDF-6 peptide corresponds to fragment 351-356 and of pigment epithelium-derived differentiation factor (PEDF), while HLDF-6 peptide corresponds to fragment 84-89 of differentiation factor HLDF isolated from HL-60 cell line. Despite high homology, these peptides had different effects on the early development. PEDF-6 had no effect on the cleavage of 2-4-cell embryos but decelerated blastocyst formation from such embryos and disturbed their structure. In the presence of HLDF-6 the blastomeres divided more actively as compared to the control and a higher number of embryos developed to the blastocyst stage. The effects of both peptides were stage-specific: the affect the embryos at early cleavage stages and, apparently, determine their further development at that moment although do not directly affect formation of the blastocysts.",

author = "Sakharova, {N. I.} and Kostanian, {I. A.} and Lepikhova, {T. N.} and Lepikhov, {K. A.} and Malashenko, {A. M.} and Navolotskaia, {E. V.} and J. Tombran-Tink and Lipkin, {V. M.}",

year = "2002",

language = "Russian",

volume = "33",

pages = "195--200",

journal = "Ontogenez",

issn = "0475-1450",

publisher = "Izdatel'stvo Nauka",

number = "3",

}

TY - JOUR

T1 - Vliianie gomologichnykh peptidov factorov differentsirovki HLDF i PEDF na doimplantatsionnoe razvitie myshei in vitro.

AU - Sakharova, N. I.

AU - Kostanian, I. A.

AU - Lepikhova, T. N.

AU - Lepikhov, K. A.

AU - Malashenko, A. M.

AU - Navolotskaia, E. V.

AU - Tombran-Tink, J.

AU - Lipkin, V. M.

PY - 2002

Y1 - 2002

N2 - We studied the effect of synthetic peptides PEDF-6 and HLDF-6 on preimplantation development of mouse embryos in vitro. PEDF-6 peptide corresponds to fragment 351-356 and of pigment epithelium-derived differentiation factor (PEDF), while HLDF-6 peptide corresponds to fragment 84-89 of differentiation factor HLDF isolated from HL-60 cell line. Despite high homology, these peptides had different effects on the early development. PEDF-6 had no effect on the cleavage of 2-4-cell embryos but decelerated blastocyst formation from such embryos and disturbed their structure. In the presence of HLDF-6 the blastomeres divided more actively as compared to the control and a higher number of embryos developed to the blastocyst stage. The effects of both peptides were stage-specific: the affect the embryos at early cleavage stages and, apparently, determine their further development at that moment although do not directly affect formation of the blastocysts.

AB - We studied the effect of synthetic peptides PEDF-6 and HLDF-6 on preimplantation development of mouse embryos in vitro. PEDF-6 peptide corresponds to fragment 351-356 and of pigment epithelium-derived differentiation factor (PEDF), while HLDF-6 peptide corresponds to fragment 84-89 of differentiation factor HLDF isolated from HL-60 cell line. Despite high homology, these peptides had different effects on the early development. PEDF-6 had no effect on the cleavage of 2-4-cell embryos but decelerated blastocyst formation from such embryos and disturbed their structure. In the presence of HLDF-6 the blastomeres divided more actively as compared to the control and a higher number of embryos developed to the blastocyst stage. The effects of both peptides were stage-specific: the affect the embryos at early cleavage stages and, apparently, determine their further development at that moment although do not directly affect formation of the blastocysts.

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M3 - Article

C2 - 12068720

AN - SCOPUS:0036561759

SN - 0475-1450

VL - 33

SP - 195

EP - 200

JO - Ontogenez

JF - Ontogenez

IS - 3

ER -

Vliianie gomologichnykh peptidov factorov differentsirovki HLDF i PEDF na doimplantatsionnoe razvitie myshei in vitro.

Abstract

All Science Journal Classification (ASJC) codes

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