TY - CHAP
T1 - Whole-genome analysis of plasmodium spp. Utilizing a new agilent technologies DNA microarray platform
AU - Painter, Heather J.
AU - Altenhofen, Lindsey M.
AU - Kafsack, Björn F.C.
AU - Llinás, Manuel
PY - 2013
Y1 - 2013
N2 - The application of DNA microarray technologies to malaria genomics has been widely used but has been limited by sample availability and technical variability. To address these issues, we present a microarray hybridization protocol that has been optimized for use with two new Agilent Technologies DNA microarrays for Plasmodium falciparum and P. berghei . Using the most recent genome sequences available for each species, we have designed ~14,000 oligonucleotide probes representing ~5,600 transcripts for each species. Included in each array design are numerous probes that allow for the identification of parasite developmental stages, common Plasmodium molecular markers used in genetic manipulation, and manufacturer probes that control for array consistency and quality. Overall, the Agilent Plasmodium spp. array designs and hybridization methodology provides a sensitive, easy-to-use, high-quality, cost-effective alternative to other currently available microarray platforms.
AB - The application of DNA microarray technologies to malaria genomics has been widely used but has been limited by sample availability and technical variability. To address these issues, we present a microarray hybridization protocol that has been optimized for use with two new Agilent Technologies DNA microarrays for Plasmodium falciparum and P. berghei . Using the most recent genome sequences available for each species, we have designed ~14,000 oligonucleotide probes representing ~5,600 transcripts for each species. Included in each array design are numerous probes that allow for the identification of parasite developmental stages, common Plasmodium molecular markers used in genetic manipulation, and manufacturer probes that control for array consistency and quality. Overall, the Agilent Plasmodium spp. array designs and hybridization methodology provides a sensitive, easy-to-use, high-quality, cost-effective alternative to other currently available microarray platforms.
UR - http://www.scopus.com/inward/record.url?scp=84934444623&partnerID=8YFLogxK
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U2 - 10.1007/978-1-62703-26-7_14
DO - 10.1007/978-1-62703-26-7_14
M3 - Chapter
C2 - 22990780
AN - SCOPUS:84934444623
SN - 9781627030250
T3 - Methods in Molecular Biology
SP - 213
EP - 219
BT - Malaria
PB - Humana Press Inc.
ER -
