Whole-genome sequence analysis reveals unique SNP profiles to distinguish vaccine and wild-type strains of bovine herpesvirus-1 (BoHV-1)

Shubhada K. Chothe; Aswathy Sebastian; Asha Thomas; Ruth H. Nissly; David Wolfgang; Maurice Byukusenge; Sunil Kumar Mor; Sagar M. Goyal; Istvan Albert; Deepanker Tewari; Bhushan M. Jayarao; Suresh V. Kuchipudi

doi:10.1016/j.virol.2018.06.015

Whole-genome sequence analysis reveals unique SNP profiles to distinguish vaccine and wild-type strains of bovine herpesvirus-1 (BoHV-1)

Shubhada K. Chothe
, Aswathy Sebastian
, Asha Thomas
, Ruth H. Nissly
, David Wolfgang
, Maurice Byukusenge
, Sunil Kumar Mor
, Sagar M. Goyal
, Istvan Albert
, Deepanker Tewari
, Bhushan M. Jayarao
, Suresh V. Kuchipudi

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Bovine herpesvirus-1 (BoHV-1) is a major pathogen affecting cattle worldwide causing primarily respiratory illness referred to as infectious bovine rhinotracheitis (IBR), along with reproductive disorders including abortion and infertility in cattle. While modified live vaccines (MLVs) effectively induce immune response against BoHV-1, they are implicated in disease outbreaks in cattle. Current diagnostic methods cannot distinguish between MLVs and field strains of BoHV-1. We performed whole genome sequencing of 18 BoHV-1 isolates from Pennsylvania and Minnesota along with five BoHV-1 vaccine strains using the Illumina Miseq platform. Based on nucleotide polymorphisms (SNPs) the sequences were clustered into three groups with two different vaccine groups and one distinct cluster of field isolates. Using this information, we developed a novel SNP-based PCR assay that can allow differentiation of vaccine and clinical strains and help accurately determine the incidence of BoHV-1 and the association of MLVs with clinical disease in cattle.

Original language	English (US)
Pages (from-to)	27-36
Number of pages	10
Journal	Virology
Volume	522
DOIs	https://doi.org/10.1016/j.virol.2018.06.015
State	Published - Sep 2018

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Virology

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10.1016/j.virol.2018.06.015

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Chothe, S. K., Sebastian, A., Thomas, A., Nissly, R. H., Wolfgang, D., Byukusenge, M., Mor, S. K., Goyal, S. M., Albert, I., Tewari, D., Jayarao, B. M., & Kuchipudi, S. V. (2018). Whole-genome sequence analysis reveals unique SNP profiles to distinguish vaccine and wild-type strains of bovine herpesvirus-1 (BoHV-1). Virology, 522, 27-36. https://doi.org/10.1016/j.virol.2018.06.015

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title = "Whole-genome sequence analysis reveals unique SNP profiles to distinguish vaccine and wild-type strains of bovine herpesvirus-1 (BoHV-1)",

abstract = "Bovine herpesvirus-1 (BoHV-1) is a major pathogen affecting cattle worldwide causing primarily respiratory illness referred to as infectious bovine rhinotracheitis (IBR), along with reproductive disorders including abortion and infertility in cattle. While modified live vaccines (MLVs) effectively induce immune response against BoHV-1, they are implicated in disease outbreaks in cattle. Current diagnostic methods cannot distinguish between MLVs and field strains of BoHV-1. We performed whole genome sequencing of 18 BoHV-1 isolates from Pennsylvania and Minnesota along with five BoHV-1 vaccine strains using the Illumina Miseq platform. Based on nucleotide polymorphisms (SNPs) the sequences were clustered into three groups with two different vaccine groups and one distinct cluster of field isolates. Using this information, we developed a novel SNP-based PCR assay that can allow differentiation of vaccine and clinical strains and help accurately determine the incidence of BoHV-1 and the association of MLVs with clinical disease in cattle.",

author = "Chothe, \{Shubhada K.\} and Aswathy Sebastian and Asha Thomas and Nissly, \{Ruth H.\} and David Wolfgang and Maurice Byukusenge and Mor, \{Sunil Kumar\} and Goyal, \{Sagar M.\} and Istvan Albert and Deepanker Tewari and Jayarao, \{Bhushan M.\} and Kuchipudi, \{Suresh V.\}",

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year = "2018",

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doi = "10.1016/j.virol.2018.06.015",

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AU - Wolfgang, David

AU - Byukusenge, Maurice

AU - Mor, Sunil Kumar

AU - Goyal, Sagar M.

AU - Albert, Istvan

AU - Tewari, Deepanker

AU - Jayarao, Bhushan M.

AU - Kuchipudi, Suresh V.

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AB - Bovine herpesvirus-1 (BoHV-1) is a major pathogen affecting cattle worldwide causing primarily respiratory illness referred to as infectious bovine rhinotracheitis (IBR), along with reproductive disorders including abortion and infertility in cattle. While modified live vaccines (MLVs) effectively induce immune response against BoHV-1, they are implicated in disease outbreaks in cattle. Current diagnostic methods cannot distinguish between MLVs and field strains of BoHV-1. We performed whole genome sequencing of 18 BoHV-1 isolates from Pennsylvania and Minnesota along with five BoHV-1 vaccine strains using the Illumina Miseq platform. Based on nucleotide polymorphisms (SNPs) the sequences were clustered into three groups with two different vaccine groups and one distinct cluster of field isolates. Using this information, we developed a novel SNP-based PCR assay that can allow differentiation of vaccine and clinical strains and help accurately determine the incidence of BoHV-1 and the association of MLVs with clinical disease in cattle.

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JF - Virology

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Whole-genome sequence analysis reveals unique SNP profiles to distinguish vaccine and wild-type strains of bovine herpesvirus-1 (BoHV-1)

Abstract

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