TY - JOUR
T1 - Whole-genome sequencing reveals resistome of highly drug-resistant retail meat and human Salmonella Dublin
AU - Eyler, Andrea B.
AU - M'ikanatha, Nkuchia M.
AU - Xiaoli, Lingzi
AU - Dudley, Edward G.
N1 - Funding Information:
The authors wish to thank Lisa Dettinger, James Tait, Carina Davis, Melinda Johnston and Barry Perry for assistance with preparation of Salmonella isolates. This work was supported by the Penn State College of Agricultural Sciences, the U.S. Food and Drug Administration grant number 1U18FD006222-01 to E.G.D., the U.S. Food and Drug Administration grant number 1U01FD006253-01 to PADOH for collaboration in the National Antimicrobial Resistance Monitoring System, Centers for Disease Control and Prevention Epidemiology and Laboratory Capacity (ELC) for collaboration in National Antimicrobial Resistance Monitoring (CDC-RFA-CI10-101204PPHF13), and the USDA National Institute of Food and Agriculture Federal Appropriations under Project PEN04522 and Accession number 0233376.
Funding Information:
The authors wish to thank Lisa Dettinger, James Tait, Carina Davis, Melinda Johnston and Barry Perry for assistance with preparation of Salmonella isolates. This work was supported by the Penn State College of Agricultural Sciences, the U.S. Food and Drug Administration grant number 1U18FD006222‐01 to E.G.D., the U.S. Food and Drug Administration grant number 1U01FD006253‐01 to PADOH for collaboration in the National Antimicrobial Resistance Monitoring System, Centers for Disease Control and Prevention Epidemiology and Laboratory Capacity (ELC) for collaboration in National Antimicrobial Resistance Monitoring (CDC‐RFA‐CI10‐101204PPHF13), and the USDA National Institute of Food and Agriculture Federal Appropriations under Project PEN04522 and Accession number 0233376.
Publisher Copyright:
© 2019 Blackwell Verlag GmbH
PY - 2020/5/1
Y1 - 2020/5/1
N2 - Non-typhoidal Salmonella (NTS) are a significant source of foodborne illness worldwide, with disease symptoms most often presenting as self-limiting gastroenteritis; however, occasionally the infection spreads and becomes invasive, frequently requiring anti-microbial treatment. The cattle-adapted Dublin serovar of NTS has commonly been associated with invasive illness and anti-microbial resistance (AMR). Here, the enhanced resolution conferred by whole-genome sequencing was utilized to elucidate and compare the resistome and genetic relatedness of 14 multidrug-resistant (MDR) and one pan-susceptible S. Dublin, isolated primarily in Pennsylvania, from fresh retail meat (one isolate) and humans (14 isolates). Twelve different genetic AMR determinants, including both acquired and chromosomal, were identified. Furthermore, comparative plasmid analysis indicated that AMR was primarily conferred by a putative IncA/C2 plasmid. A single pan-susceptible S. Dublin isolate, collected from the same timeframe and geographical region as the MDR isolates, did not carry an IncA/C2 replicon sequence within its genome. Moreover, the pan-susceptible isolate was genetically distinct from its MDR counterparts, as it was separated by ≥267 single nucleotide polymorphisms (SNPs), whereas there was a ≤38 SNP distance between the MDR isolates. Collectively, this data set advances our understanding of the genetic basis of the highly drug-resistant nature of S. Dublin, a serovar with significant public health implications.
AB - Non-typhoidal Salmonella (NTS) are a significant source of foodborne illness worldwide, with disease symptoms most often presenting as self-limiting gastroenteritis; however, occasionally the infection spreads and becomes invasive, frequently requiring anti-microbial treatment. The cattle-adapted Dublin serovar of NTS has commonly been associated with invasive illness and anti-microbial resistance (AMR). Here, the enhanced resolution conferred by whole-genome sequencing was utilized to elucidate and compare the resistome and genetic relatedness of 14 multidrug-resistant (MDR) and one pan-susceptible S. Dublin, isolated primarily in Pennsylvania, from fresh retail meat (one isolate) and humans (14 isolates). Twelve different genetic AMR determinants, including both acquired and chromosomal, were identified. Furthermore, comparative plasmid analysis indicated that AMR was primarily conferred by a putative IncA/C2 plasmid. A single pan-susceptible S. Dublin isolate, collected from the same timeframe and geographical region as the MDR isolates, did not carry an IncA/C2 replicon sequence within its genome. Moreover, the pan-susceptible isolate was genetically distinct from its MDR counterparts, as it was separated by ≥267 single nucleotide polymorphisms (SNPs), whereas there was a ≤38 SNP distance between the MDR isolates. Collectively, this data set advances our understanding of the genetic basis of the highly drug-resistant nature of S. Dublin, a serovar with significant public health implications.
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U2 - 10.1111/zph.12680
DO - 10.1111/zph.12680
M3 - Article
C2 - 31867871
AN - SCOPUS:85076890441
SN - 1863-1959
VL - 67
SP - 251
EP - 262
JO - Zoonoses and Public Health
JF - Zoonoses and Public Health
IS - 3
ER -
