Wiring an [FeFe]-hydrogenase with Photosystem i for light-induced hydrogen production

Carolyn E. Lubner; Philipp Knörzer; Paulo J.N. Silva; Kylie A. Vincent; Thomas Happe; Donald A. Bryant; John H. Golbeck

doi:10.1021/bi1016167

Wiring an [FeFe]-hydrogenase with Photosystem i for light-induced hydrogen production

Carolyn E. Lubner, Philipp Knörzer, Paulo J.N. Silva, Kylie A. Vincent, Thomas Happe, Donald A. Bryant, John H. Golbeck

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

A molecular wire is used to connect two proteins through their physiologically relevant redox cofactors to facilitate direct electron transfer. Photosystem I (PS I) and an [FeFe]-hydrogenase (H₂ase) serve as the test bed for this new technology. By tethering a photosensitizer with a hydrogen-evolving catalyst, attached by Fe-S coordination bonds between the FB iron-sulfur cluster of PS I and the distal iron-sulfur cluster of H ₂ase, we assayed electron transfer between the two components via light-induced hydrogen generation. These hydrogen-producing nanoconstructs self-assemble when the PS I variant, the H₂ase variant, and the molecular wire are combined.

Original language	English (US)
Pages (from-to)	10264-10266
Number of pages	3
Journal	Biochemistry
Volume	49
Issue number	48
DOIs	https://doi.org/10.1021/bi1016167
State	Published - Dec 7 2010

All Science Journal Classification (ASJC) codes

Biochemistry

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10.1021/bi1016167

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title = "Wiring an [FeFe]-hydrogenase with Photosystem i for light-induced hydrogen production",

abstract = "A molecular wire is used to connect two proteins through their physiologically relevant redox cofactors to facilitate direct electron transfer. Photosystem I (PS I) and an [FeFe]-hydrogenase (H2ase) serve as the test bed for this new technology. By tethering a photosensitizer with a hydrogen-evolving catalyst, attached by Fe-S coordination bonds between the FB iron-sulfur cluster of PS I and the distal iron-sulfur cluster of H 2ase, we assayed electron transfer between the two components via light-induced hydrogen generation. These hydrogen-producing nanoconstructs self-assemble when the PS I variant, the H2ase variant, and the molecular wire are combined.",

author = "Lubner, {Carolyn E.} and Philipp Kn{\"o}rzer and Silva, {Paulo J.N.} and Vincent, {Kylie A.} and Thomas Happe and Bryant, {Donald A.} and Golbeck, {John H.}",

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T1 - Wiring an [FeFe]-hydrogenase with Photosystem i for light-induced hydrogen production

AU - Lubner, Carolyn E.

AU - Knörzer, Philipp

AU - Silva, Paulo J.N.

AU - Vincent, Kylie A.

AU - Happe, Thomas

AU - Bryant, Donald A.

AU - Golbeck, John H.

PY - 2010/12/7

Y1 - 2010/12/7

N2 - A molecular wire is used to connect two proteins through their physiologically relevant redox cofactors to facilitate direct electron transfer. Photosystem I (PS I) and an [FeFe]-hydrogenase (H2ase) serve as the test bed for this new technology. By tethering a photosensitizer with a hydrogen-evolving catalyst, attached by Fe-S coordination bonds between the FB iron-sulfur cluster of PS I and the distal iron-sulfur cluster of H 2ase, we assayed electron transfer between the two components via light-induced hydrogen generation. These hydrogen-producing nanoconstructs self-assemble when the PS I variant, the H2ase variant, and the molecular wire are combined.

AB - A molecular wire is used to connect two proteins through their physiologically relevant redox cofactors to facilitate direct electron transfer. Photosystem I (PS I) and an [FeFe]-hydrogenase (H2ase) serve as the test bed for this new technology. By tethering a photosensitizer with a hydrogen-evolving catalyst, attached by Fe-S coordination bonds between the FB iron-sulfur cluster of PS I and the distal iron-sulfur cluster of H 2ase, we assayed electron transfer between the two components via light-induced hydrogen generation. These hydrogen-producing nanoconstructs self-assemble when the PS I variant, the H2ase variant, and the molecular wire are combined.

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Wiring an [FeFe]-hydrogenase with Photosystem i for light-induced hydrogen production

Abstract

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