Wnt signaling directs human pluripotent stem cells into vascularized cardiac organoids with chamber-like structures

Po Yu Liang, Yun Chang, Gyuhyung Jin, Xiaojun Lian, Xiaoping Bao

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Heart diseases are leading cause of death around the world. Given their unique capacity to self-renew and differentiate into all types of somatic cells, human pluripotent stem cells (hPSCs) hold great promise for heart disease modeling and cardiotoxic drug screening. hPSC-derived cardiac organoids are emerging biomimetic models for studying heart development and cardiovascular diseases, but it remains challenging to make mature organoids with a native-like structure in vitro. In this study, temporal modulation of Wnt signaling pathway co-differentiated hPSCs into beating cardiomyocytes and cardiac endothelial-like cells in 3D organoids, resulting in cardiac endothelial-bounded chamber formation. These chambered cardiac organoids exhibited more mature membrane potential compared to cardiac organoids composed of only cardiomyocytes. Furthermore, a better response to toxic drugs was observed in chamber-contained cardiac organoids. In summary, spatiotemporal signaling pathway modulation may lead to more mature cardiac organoids for studying cardiovascular development and diseases.

Original languageEnglish (US)
Article number1059243
JournalFrontiers in Bioengineering and Biotechnology
Volume10
DOIs
StatePublished - Nov 18 2022

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Histology
  • Biomedical Engineering

Fingerprint

Dive into the research topics of 'Wnt signaling directs human pluripotent stem cells into vascularized cardiac organoids with chamber-like structures'. Together they form a unique fingerprint.

Cite this