WrbA from Escherichia coli and Archaeoglobus fulgidus is an NAD(P)H:quinone oxidoreductase

Eric V. Patridge, James G. Ferry

Research output: Contribution to journalArticlepeer-review

103 Scopus citations


WrbA (tryptophan [W] repressor-binding protein) was discovered in Escherichia coli, where it was proposed to play a role in regulation of the tryptophan operon; however, this has been put in question, leaving the function unknown. Here we report a phylogenetic analysis of 30 sequences which indicated that WrbA is the prototype of a distinct family of flavoproteins which exists in a diversity of cell types across all three domains of life and includes documented NAD(P)H:quinone oxidoreductases (NQOs) from the Fungi and Viridiplantae kingdoms. Biochemical characterization of the prototypic WrbA protein from E. coli and WrbA from Archaeoglobus fulgidus, a hyperthermophilic species from the Archaea domain, shows that these enzymes have NQO activity, suggesting that this activity is a defining characteristic of the WrbA family that we designate a new type of NQO (type IV). For E. coli WrbA, the K mNADH was 14 ± 0.43 μM and the K mbenzoquinone was 5.8 ± 0.12 μM. For A. fulgidus WrbA, the KmNADH was 19 ± 1.7 μM and the K mbenzoquinone was 37 ± 3.6 μM. Both enzymes were found to be homodimeric by gel filtration chromatography and homotetrameric by dynamic light scattering and to contain one flavin mononucleotide molecule per monomer. The NQO activity of each enzyme is retained over a broad pH range, and apparent initial velocities indicate that maximal activities are comparable to the optimum growth temperature for the respective organisms. The results are discussed and implicate WrbA in the two-electron reduction of quinones, protecting against oxidative stress.

Original languageEnglish (US)
Pages (from-to)3498-3506
Number of pages9
JournalJournal of bacteriology
Issue number10
StatePublished - May 2006

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology


Dive into the research topics of 'WrbA from Escherichia coli and Archaeoglobus fulgidus is an NAD(P)H:quinone oxidoreductase'. Together they form a unique fingerprint.

Cite this