TY - JOUR
T1 - Xenobiotic microsomal epoxide hydrolase
T2 - 5′ sequence of the human gene
AU - Wilson, Neil M.
AU - Omiecinski, Curtis J.
N1 - Funding Information:
verified with sequence determinations from independently isolated genomic clones. When the 5' flanking regions of the human and rat \[2\] mEH genes were compared, only 47~ overall homology was observed. This is in contrast to the \[~3~s equen~ conservation for the respective cDNAs. A 20 base antisense ofigomer directed to the 5' part (see Fig. 1) of the human mEH eDNA was synthesized and used to carry out primer extension analysis with poly(A) + RNA isolated from a human fetal fiver. As shown in Fig. 2, a single band was detected. Whereas rat mEH transcription begins at an A residue \[2\]t,h e human mEH gene initiates at a C residue. Of note, the transcription initiation site in the human mEH gene was 116 nucleotides upstream of the analogous site in the rat gene. A CAAT box was not identified in tile human gene. However, an analogous CTF-binding element, identical to that found in the human alpha 1-globin gene \[3\]w, as located further upstream (position -278). An SV40 core enhancer motif \[4\]w as also apparent at position -525 of the human gene. The isolation of the human mEH gene will permit detailed analysis of the molecular events involved in regulating its expression in human tissues. This work was supported by NIH grants ES04978, ES05374, and a grant from the Charles A. Dana Foundation. The authors thank Dr. A. Ullrich of the Genen-
PY - 1989/8/14
Y1 - 1989/8/14
N2 - A cDNA for human microsomal epoxide hydrolase (mEH) was used to isolate genomic clones representing the 5′ portion of the corresponding human gene. A total of 1034 bases of mEH gene sequence were obtained that included exon 1 and 5′ flanking information. In contrast to the structure reported for the rat mEH gene (Falany et al. (1987) J. Biol. Chem. 262, 5924-5930), the human mEH gene lacked at CAAT box, but did contain a high-affinity CTF binding site at position -278 and an SV40 core enhancer element at position -525. TATA boxes were identified at positions -32 and -29 for the human and rat genes, respectively. Sequence comparisons between 5′ flanking regions of the human and rat mEH genes demonstrated 47% homology, considerably less than the 83% conservation observed in the cDNA coding areas. Primer extension experiments localized the transcription initiation site of the human gene to a position 116 bases 5′ from the analogous site in the rat mEH gene.
AB - A cDNA for human microsomal epoxide hydrolase (mEH) was used to isolate genomic clones representing the 5′ portion of the corresponding human gene. A total of 1034 bases of mEH gene sequence were obtained that included exon 1 and 5′ flanking information. In contrast to the structure reported for the rat mEH gene (Falany et al. (1987) J. Biol. Chem. 262, 5924-5930), the human mEH gene lacked at CAAT box, but did contain a high-affinity CTF binding site at position -278 and an SV40 core enhancer element at position -525. TATA boxes were identified at positions -32 and -29 for the human and rat genes, respectively. Sequence comparisons between 5′ flanking regions of the human and rat mEH genes demonstrated 47% homology, considerably less than the 83% conservation observed in the cDNA coding areas. Primer extension experiments localized the transcription initiation site of the human gene to a position 116 bases 5′ from the analogous site in the rat mEH gene.
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U2 - 10.1016/0167-4781(89)90029-8
DO - 10.1016/0167-4781(89)90029-8
M3 - Article
C2 - 2758034
AN - SCOPUS:0024343432
SN - 0167-4781
VL - 1008
SP - 357
EP - 358
JO - BBA - Gene Structure and Expression
JF - BBA - Gene Structure and Expression
IS - 3
ER -