TY - JOUR
T1 - XSWR-C and INO80 chromatin remodelers recognize nucleosome-free regions near +1 nucleosomes
AU - Yen, Kuangyu
AU - Vinayachandran, Vinesh
AU - Pugh, B. Franklin
N1 - Funding Information:
We thank Matt Rossi for valuable suggestions on the figures, Anna Chan-Salis for sequencing, and Yunfei Li for bioinformatics support. This work was funded by NIH grant 5R01HG4160.
PY - 2013/9/12
Y1 - 2013/9/12
N2 - SWR-C/SWR1 and INO80 are multisubunit complexes that catalyze the deposition and removal, respectively, of histone variant H2A.Z from the first nucleosome at the start of genes. How they target and engage these +1 nucleosomes is unclear. Using ChIP-exo, we identified the subnucleosomal placement of 20 of their subunits across the yeast genome. The Swc2 subunit of SWR-C bound a narrowly defined region in the adjacent nucleosome-free region (NFR), where it positioned the Swr1 subunit over one of two sites of H2A.Z deposition at +1. The genomic binding maps suggest that many subunits have a rather plastic organization that allows subunits to exchange between the two complexes. One outcome of promoting H2A/H2A.Z exchange was an enhanced turnover of entire nucleosomes, thereby creating dynamic chromatin at the start of genes. Our findings provide unifying concepts on how these two opposing chromatin remodeling complexes function selectively at the +1 nucleosome of nearly all genes.
AB - SWR-C/SWR1 and INO80 are multisubunit complexes that catalyze the deposition and removal, respectively, of histone variant H2A.Z from the first nucleosome at the start of genes. How they target and engage these +1 nucleosomes is unclear. Using ChIP-exo, we identified the subnucleosomal placement of 20 of their subunits across the yeast genome. The Swc2 subunit of SWR-C bound a narrowly defined region in the adjacent nucleosome-free region (NFR), where it positioned the Swr1 subunit over one of two sites of H2A.Z deposition at +1. The genomic binding maps suggest that many subunits have a rather plastic organization that allows subunits to exchange between the two complexes. One outcome of promoting H2A/H2A.Z exchange was an enhanced turnover of entire nucleosomes, thereby creating dynamic chromatin at the start of genes. Our findings provide unifying concepts on how these two opposing chromatin remodeling complexes function selectively at the +1 nucleosome of nearly all genes.
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U2 - 10.1016/j.cell.2013.08.043
DO - 10.1016/j.cell.2013.08.043
M3 - Article
C2 - 24034248
AN - SCOPUS:84884234697
SN - 0092-8674
VL - 154
SP - 1246
JO - Cell
JF - Cell
IS - 6
ER -