Yeast TAF(II)145 required for transcription of G1/S cyclin genes and regulated by the cellular growth state

Scott S. Walker; Wu Cheng Shen; Joseph C. Reese; Lynne M. Apone; Michael R. Green

doi:10.1016/S0092-8674(00)80522-X

Yeast TAF(II)145 required for transcription of G1/S cyclin genes and regulated by the cellular growth state

Scott S. Walker, Wu Cheng Shen, Joseph C. Reese, Lynne M. Apone, Michael R. Green

Research output: Contribution to journal › Article › peer-review

129 Scopus citations

Abstract

TFIID comprises the TATA box-binding protein and a set of highly conserved associated factors (TAF(II)S). yTAF(II)145, the core subunit of the yeast TAF(II) complex, is dispensable for transcription of most yeast genes but specifically required for progression through G1/S. Here we show that transcription of G1 and certain B-type cyclin genes is dependent upon yTAF(II)145. At high cell density or following nutrient deprivation, yeast cells cease division, enter a G₀-like state, and terminate transcription of most genes. In this stationary phase, we find that the levels of yTAF(II)145, several other yTAF(II)S, and TBP are drastically reduced. Collectively, our results indicate that yTAF(II)145 and other TF(II)D components have a specialized role in transcriptional regulation of cell cycle progression and growth control.

Original language	English (US)
Pages (from-to)	607-614
Number of pages	8
Journal	Cell
Volume	90
Issue number	4
DOIs	https://doi.org/10.1016/S0092-8674(00)80522-X
State	Published - Aug 22 1997

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology

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10.1016/S0092-8674(00)80522-X

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@article{b11a5cc8fefa4ae3b32bf5e3f2e7e209,

title = "Yeast TAF(II)145 required for transcription of G1/S cyclin genes and regulated by the cellular growth state",

abstract = "TFIID comprises the TATA box-binding protein and a set of highly conserved associated factors (TAF(II)S). yTAF(II)145, the core subunit of the yeast TAF(II) complex, is dispensable for transcription of most yeast genes but specifically required for progression through G1/S. Here we show that transcription of G1 and certain B-type cyclin genes is dependent upon yTAF(II)145. At high cell density or following nutrient deprivation, yeast cells cease division, enter a G0-like state, and terminate transcription of most genes. In this stationary phase, we find that the levels of yTAF(II)145, several other yTAF(II)S, and TBP are drastically reduced. Collectively, our results indicate that yTAF(II)145 and other TF(II)D components have a specialized role in transcriptional regulation of cell cycle progression and growth control.",

author = "Walker, {Scott S.} and Shen, {Wu Cheng} and Reese, {Joseph C.} and Apone, {Lynne M.} and Green, {Michael R.}",

note = "Funding Information: We thank I. Herskowitz, B. Andrews and members of the lab for reagents, helpful discussions, and advice; A. Amon for insightful comments on the manuscript; D. Shore and K. Nasmyth for advice on the stationary phase in yeast; M. Woda and the University of Massachusetts Medical Center Flow Cytology Laboratory for FACS analysis; and J. Keilty for technical assistance. S. S. W. was supported by a senior postdoctoral fellowship from the American Cancer Society (Massachusetts Division). J. C. R. was supported by a Damon Runyon-Walter Winchell Cancer Research Foundation Fellowship. W.-C. S. is a postdoctoral fellow and M. R. G. is an investigator of the Howard Hughes Medical Institute. This work was supported in part by a grant from the National Institutes of Health to M. R. G.",

year = "1997",

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T1 - Yeast TAF(II)145 required for transcription of G1/S cyclin genes and regulated by the cellular growth state

AU - Walker, Scott S.

AU - Shen, Wu Cheng

AU - Reese, Joseph C.

AU - Apone, Lynne M.

AU - Green, Michael R.

N1 - Funding Information: We thank I. Herskowitz, B. Andrews and members of the lab for reagents, helpful discussions, and advice; A. Amon for insightful comments on the manuscript; D. Shore and K. Nasmyth for advice on the stationary phase in yeast; M. Woda and the University of Massachusetts Medical Center Flow Cytology Laboratory for FACS analysis; and J. Keilty for technical assistance. S. S. W. was supported by a senior postdoctoral fellowship from the American Cancer Society (Massachusetts Division). J. C. R. was supported by a Damon Runyon-Walter Winchell Cancer Research Foundation Fellowship. W.-C. S. is a postdoctoral fellow and M. R. G. is an investigator of the Howard Hughes Medical Institute. This work was supported in part by a grant from the National Institutes of Health to M. R. G.

PY - 1997/8/22

Y1 - 1997/8/22

N2 - TFIID comprises the TATA box-binding protein and a set of highly conserved associated factors (TAF(II)S). yTAF(II)145, the core subunit of the yeast TAF(II) complex, is dispensable for transcription of most yeast genes but specifically required for progression through G1/S. Here we show that transcription of G1 and certain B-type cyclin genes is dependent upon yTAF(II)145. At high cell density or following nutrient deprivation, yeast cells cease division, enter a G0-like state, and terminate transcription of most genes. In this stationary phase, we find that the levels of yTAF(II)145, several other yTAF(II)S, and TBP are drastically reduced. Collectively, our results indicate that yTAF(II)145 and other TF(II)D components have a specialized role in transcriptional regulation of cell cycle progression and growth control.

AB - TFIID comprises the TATA box-binding protein and a set of highly conserved associated factors (TAF(II)S). yTAF(II)145, the core subunit of the yeast TAF(II) complex, is dispensable for transcription of most yeast genes but specifically required for progression through G1/S. Here we show that transcription of G1 and certain B-type cyclin genes is dependent upon yTAF(II)145. At high cell density or following nutrient deprivation, yeast cells cease division, enter a G0-like state, and terminate transcription of most genes. In this stationary phase, we find that the levels of yTAF(II)145, several other yTAF(II)S, and TBP are drastically reduced. Collectively, our results indicate that yTAF(II)145 and other TF(II)D components have a specialized role in transcriptional regulation of cell cycle progression and growth control.

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Yeast TAF(II)145 required for transcription of G1/S cyclin genes and regulated by the cellular growth state

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