TY - JOUR
T1 - Zearalenone induced toxicity in SHSY-5Y cells
T2 - The role of oxidative stress evidenced by N-acetyl cysteine
AU - Venkataramana, M.
AU - Chandra Nayaka, S.
AU - Anand, T.
AU - Rajesh, Rajaiah
AU - Aiyaz, Mohammed
AU - Divakara, S. T.
AU - Murali, H. S.
AU - Prakash, H. S.
AU - Lakshmana Rao, P. V.
PY - 2014/3
Y1 - 2014/3
N2 - Zearalenone (ZEN) is a mycotoxin from Fusarium species commonly found in many food commodities and are known to cause reproductive disorders, genotoxic and immunosuppressive effects. Although many studies have demonstrated the cytotoxic effects of ZEN, the mechanisms by which ZEN mediates its cytotoxic effects appear to differ according to cell type and route of exposure. Meantime, the available information on the neurotoxic effects of ZEN is very much limited. In the present study we evaluated the role of oxidative stress in ZEN mediated neurotoxicity in SH-SY5Y cells and investigated the possible underlying mechanism. ZEN induced ROS formation and elevated levels of MDA, loss of mitochondrial membrane potential (MMP) and increase in DNA damage in a dose dependent manner as assessed by COMET assay and agarose gel electrophoresis. However, there was no DNA damage by plasmid breakage assay at 6, 12 and 24. h time points. DAPI staining showed apoptotic nuclei at 12 and 24. h. Further, ZEN treated SH-SY5Y cells showed a marked suppressive effect on the neuronal gene expression. Use of an antioxidant N-acetylcysteine (NAC) reversed the toxin-induced generation of ROS and also attenuated loss of MMP. Collectively, these results suggest that ROS is the main upstream signal leading to increased ZEN mediated neurotoxicity in SH-SY5Y cells.
AB - Zearalenone (ZEN) is a mycotoxin from Fusarium species commonly found in many food commodities and are known to cause reproductive disorders, genotoxic and immunosuppressive effects. Although many studies have demonstrated the cytotoxic effects of ZEN, the mechanisms by which ZEN mediates its cytotoxic effects appear to differ according to cell type and route of exposure. Meantime, the available information on the neurotoxic effects of ZEN is very much limited. In the present study we evaluated the role of oxidative stress in ZEN mediated neurotoxicity in SH-SY5Y cells and investigated the possible underlying mechanism. ZEN induced ROS formation and elevated levels of MDA, loss of mitochondrial membrane potential (MMP) and increase in DNA damage in a dose dependent manner as assessed by COMET assay and agarose gel electrophoresis. However, there was no DNA damage by plasmid breakage assay at 6, 12 and 24. h time points. DAPI staining showed apoptotic nuclei at 12 and 24. h. Further, ZEN treated SH-SY5Y cells showed a marked suppressive effect on the neuronal gene expression. Use of an antioxidant N-acetylcysteine (NAC) reversed the toxin-induced generation of ROS and also attenuated loss of MMP. Collectively, these results suggest that ROS is the main upstream signal leading to increased ZEN mediated neurotoxicity in SH-SY5Y cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=84893382960&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2013.12.042
DO - 10.1016/j.fct.2013.12.042
M3 - Article
C2 - 24412706
AN - SCOPUS:84893382960
SN - 0278-6915
VL - 65
SP - 335
EP - 342
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
ER -