TY - GEN
T1 - Zinc oxide nanorod integrated microdevice for multiplex virus detection
AU - Yu, Xu
AU - Xia, Yiqiu
AU - Tang, Yi
AU - Zhang, Wen Long
AU - Yeh, Yin Ting
AU - Lu, Huaguang
AU - Zheng, Si Yang
N1 - Publisher Copyright:
© 2017 IEEE.
PY - 2017/7/26
Y1 - 2017/7/26
N2 - Technology development for point-of-care viral pathogen detection is critical for early diagnosis of infectious diseases, and rapid and effective disease intervention. In this paper, we present the development of a zinc oxide nanorod-integrated microdevice for highly sensitive and specific detection of avian influenza virus. This multiplexed immunofluorescence platform takes two advantages of the zinc oxide nanorods. On one hand, the 3D morphology of zinc oxide nanorods efficiently increases the effective surface area for monoclonal antibodies and decreases the diffusion distance between antibody and pathogens. On the other hand, the unique optical property of the translucent randomly ordered zinc oxide nanorod surface enhances fluorescence detection by 30-70%. We demonstrated the detection limit of the H5N2 avian influenza virus could be lowered down to 3.6×103 EID50/mL (EID50: 50% embryo infectious dose), which was about 22 times more sensitive than conventional ELISA assay tested under the same conditions. We further designed the microfluidic biosensor platform to detect multiple viruses simultaneously by spatial encoding of capture antibodies. One prominent feature of the device is that the captured H5N2 avian influenza virus can be released by simply dissolving zinc oxide nanorods under slightly acidic environment for subsequent off-chip analyses. As a whole, this platform provides a powerful tool for rapid detection of multiple pathogens, which may extent to the other fields for low-cost and convenient biomarker detection.
AB - Technology development for point-of-care viral pathogen detection is critical for early diagnosis of infectious diseases, and rapid and effective disease intervention. In this paper, we present the development of a zinc oxide nanorod-integrated microdevice for highly sensitive and specific detection of avian influenza virus. This multiplexed immunofluorescence platform takes two advantages of the zinc oxide nanorods. On one hand, the 3D morphology of zinc oxide nanorods efficiently increases the effective surface area for monoclonal antibodies and decreases the diffusion distance between antibody and pathogens. On the other hand, the unique optical property of the translucent randomly ordered zinc oxide nanorod surface enhances fluorescence detection by 30-70%. We demonstrated the detection limit of the H5N2 avian influenza virus could be lowered down to 3.6×103 EID50/mL (EID50: 50% embryo infectious dose), which was about 22 times more sensitive than conventional ELISA assay tested under the same conditions. We further designed the microfluidic biosensor platform to detect multiple viruses simultaneously by spatial encoding of capture antibodies. One prominent feature of the device is that the captured H5N2 avian influenza virus can be released by simply dissolving zinc oxide nanorods under slightly acidic environment for subsequent off-chip analyses. As a whole, this platform provides a powerful tool for rapid detection of multiple pathogens, which may extent to the other fields for low-cost and convenient biomarker detection.
UR - http://www.scopus.com/inward/record.url?scp=85029381852&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029381852&partnerID=8YFLogxK
U2 - 10.1109/TRANSDUCERS.2017.7994182
DO - 10.1109/TRANSDUCERS.2017.7994182
M3 - Conference contribution
AN - SCOPUS:85029381852
T3 - TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
SP - 850
EP - 853
BT - TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
Y2 - 18 June 2017 through 22 June 2017
ER -