ZO-1 tight junction protein expression in rat retinas

S. A. Khin, A. J. Barber, T. W. Gardner

Research output: Contribution to journalArticlepeer-review


Purpose: To determine ZO-1 tight junction protein distribution in control and diabetic rat retinas. Methods: Eighteen of 28 male Sprague-Dawley rats were made diabetic by IV streptozotocin injection, 65 mg/kg. Animals were sacrificed after 7 or 12 weeks of diabetes and the eyes enucleated. ZO-1 indirect immunofluorescence was examined in cryostat sections with a monoclonal antibody. Endothelium was localized with a polyclonal anti-von Willebrand factor antibody, ZO-1 immunoreactivity was estimated by masked evaluation. Results: ZO-1 immunofluorescence was found in vWF-positive microvessels in retina and choroid. ZO-1 immunofluorescence was not detected in the retinal pigment epithelium. ZO-1 immunoreactivity was judged to be reduced in 14/18 diabetic animals versus 3/10 control animals. Conclusions: ZO-1 protein is found in the inner blood-retinal barrier of rats. Experimental diabetes may reduce ZO-1 content and contribute to increased blood-retinal barrier permeability.

Original languageEnglish (US)
Pages (from-to)S971
JournalInvestigative Ophthalmology and Visual Science
Issue number3
StatePublished - Feb 15 1996

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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